【作者】 桂雨婷； 王健； 余建波； 李德亮；

【Author】 Yuting Gui;Jian Wang;Jianbo Yu;Deliang Li;Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, College of Animal Science and Technology, Hunan Agricultural University;

【机构】 湖南农业大学动物科学技术学院湖南省特色水产资源利用工程技术研究中心；

【摘要】 本研究旨在分析低浓度镉（0.01mg/L）污染水体中添加钙对草鱼的保护作用及其机制。实验设置对照（0 mg/L）、低钙（30 mg/L）、中钙（60 mg/L）和高钙（90 mg/L）四个组,历时30天。结果显示:添加Ca²⁺对草鱼生长并无显著影响（p>0.05）。所有实验组草鱼组织Cd富集量顺序均为肾>肠>鳃>肝>肌肉≈血液,且肾脏和鳃Cd富集量随Ca²⁺浓度的增加呈显著下降趋势（p<0.05）。Ca²⁺显著降低了草鱼血浆Na⁺和Cl^-浓度。中钙组草鱼肝脏POD、CAT和T-SOD活性均显著低于其他实验组（p<0.05）,高钙组草鱼肝脏MDA含量显著高于其他组（p<0.05）;中钙组和高钙组草鱼肌肉中POD、CAT、T-SOD活性均显著低于其他实验组（p<0.05）,MDA含量无显著差异（p>0.05）。鳃Na⁺/K⁺-ATPase、Ca²⁺-ATPase活性变化在各组间的差异变化显著（p<0.05）。高钙组草鱼鳃Na⁺/K⁺-ATPase和Ca²⁺-ATPase基因表达量显著高于其他实验组（p<0.05）。研究结果表明,外源钙添加对镉污染水体草鱼具有一定的保护作用。更多还原

【Abstract】 In order to analyze the protective effects and mechanism of Ca²⁺ on Cd toxicology in grass carp（Ctenopharyngodon idella） in water polluted with low Cd（0.01 mg/L）. The experiment was conducted in four groups with control（0 mg/L）, low（30 mg/L）, medium（60 mg/L）, and high Ca²⁺（90 mg/L） for 30 days. The results showed that different concentrations of Ca²⁺ had no significant effect on grass carp growth（p > 0.05）. The Cd bioaccumulation level in all examined tissues were kidney>intestinal>gill>liver>muscle≈blood. The Cd concentration in kidney and gill decreased significantly with the increase of Ca²⁺ concentration（p<0.05）, and Ca²⁺ significantly reduced the plasma Na+ and Cl-concentrations（p<0.05）. The activities of POD, CAT and T-SOD in the liver of the medium Ca group were significantly lower than those in other groups（p<0.05）, and the MDA content in the liver of the high Ca²⁺ group was significantly higher than that in other groups（p<0.05）. The activities of POD, CAT and T-SOD in the muscles of the middle Ca²⁺ group and the MDA contents in muscle of high Ca²⁺ group were significantly higher than other groups（p<0.05）. The activities of Na⁺/K⁺-ATPase and Ca2+-ATPase in the gill of grass carp was significantly different among treatments（p<0.05）. The m RNA expression levels of gill Na⁺/K⁺-ATPase and Ca2+-ATPase genes were the highest in high Ca²⁺ group. The results showed that the addition of exogenous Ca had a certain protective effects on the toxicity of Cd to grass carp.更多还原