【作者】 蔡煜； 刘东超； 陈仁基； 谢建华； 吴靖匀；

【Author】 CAI yu;LIU Dongchao;CHEN Renji;XIE Jianhua;WU Jingyun;Fisheries College of Guangdong Ocean University;Engineering Technology Research Center for Algae Breeding and Application,Guangdong Province;

【机构】 广东海洋大学水产学院； 广东省藻类养殖及应用工程技术中心；

【摘要】 为了培养出高附加值的富硒长茎葡萄蕨藻（Caulerpa lentillifera）,本实验以亚硒酸钠（Na₂SeO₃）为硒源,研究了不同硒浓度对长茎葡萄蕨藻的生长、光合色素和硒富集量的影响,并设计正交实验确定适宜长茎葡萄蕨藻富硒的最佳温度、光照和N/P比。结果表明:1)随着硒浓度的增加,藻体内硒的富集量显著性增加（P<0.05）;当硒浓度大于2 mg/L时,显著抑制藻体的生长（P<0.05）;因此,2 mg/L的亚硒酸钠适合培养富硒长茎葡萄蕨藻。2)长茎葡萄蕨藻生长和富硒的最适温度范围为24～28℃,最适光照范围为2000～6000 lx,最适N/P比范围为5:1～10:1,其中温度24℃、照度2000 lx、N/P比8:1是培养富硒长茎葡萄蕨藻的最优组合条件。更多还原

【Abstract】 In order to cultivate selenium-rich Caulerpa lentillifera, this experiment used sodium selenite（Na₂SeO₃） as the source of selenium, studyded different selenium concentrations on the growth, photosynthetic pigment and selenium concentration of C. lentillifera, and the optimum temperature, light and N/P ratio were determined by orthogonal experimental design. The results showed that: 1) with the increase of selenium concentration, the enrichment of selenium in algae increased significantly（P < 0.05）. When selenium concentration was higher than 2 mg/L, the growth of algae was significantly inhibited（P < 0.05）. Therefore, 2 mg/L was the most suitable for cultivation. 2) The optimum temperature range for the growth and selenium enrichment of C. lentillifera was 24 ～ 28 °C, the optimum illumination was 2000～ 6000 lx, and the optimum N/P ratio was 5:1～10:1, of which the temperature was 24 °C, the illumination was 2000 lx and the N/P ratio of 8:1 was the optimal combination condition for cultivating C. lentillifera.更多还原