【作者】 连跃斌； 许凯； 王文磊； 徐燕； 纪德华； 陈昌生； 谢潮添；

【Author】 LIAN Yuebin;XU Kai;WANG Wenlei;XU Yan;JI Dehua;CHEN Changsheng;XIE Chaotian;College of Fisheries, Jimei University;

【机构】 集美大学水产学院；

【摘要】 本研究以六种不同颜色坛紫菜(Pyropia haitanensis)品系为研究材料,通过生理和分子生物学手段,研究坛紫菜颜色的形成机制。根据色度值将6个品系划分为红黄组和绿组,红黄组包括三个色素突变品系(红色、橘黄色和紫色)和一个野生品系(野生色),绿组包括两个色素突变品系(棕绿色和翠绿色)。红黄组品系的平均藻红蛋白(PE)含量比绿组高1倍,但两组在藻蓝蛋白(PC)和别藻蓝蛋白(APC)含量上没有显著差异,而红黄组的平均Chl a含量显著比绿组低35%,这导致红黄组的PE/Chl a比值上比绿组高1.7倍。红黄组的藻胆蛋白合成关键基因原卟啉亚铁螯合酶(hem H)、血红素氧化酶(HMOX1)和铁氧还蛋白氧化还原酶(HY2)比绿组表达水平高,但两组在叶绿素合成关键基因谷氨酰-tRNA还原酶(hemA)和Mg-鳌合酶(CHLH、CHL1、CHLD)的表达上没有显著差异。此外,红黄组的藻胆蛋白相关代谢物含量比绿组高,而叶绿素相关代谢物含量则比绿组低。以上研究结果表明,坛紫菜藻胆蛋白合成关键基因的差异表达,导致藻红蛋白和叶绿素含量的组间差异,最终导致了藻体颜色的差异。更多还原

【Abstract】 In this study, to investigate the color formation mechanism of pigment mutants of Pyropia haitanensis, six strains of Pyropia haitanensis with different color were used as research materials. Base on the chroma values, six strains were divided into red-yellow group and green group. The red-yellow group includes three pigment mutant strains(Red, Orange and Purple) and a wild strain(Wild color), The green group includes two pigment mutant strains(Brown-green and Olive-drab). The average content of phycoerythrin(PE) in the red-yellow group was one time higher than the green group, but there were no significant differences in the content of phycocyanin(PC) and allophycocyanin(APC) between the two groups, while the average content of Chl a in the red-yellow group was 35% lower than the green group. Consequently, the ratio of PE/Chl a in the red-yellow group was 1.7 times higher than the green group. For red-yellow group, its key genes involved in phycobiliprotein synthesis, including protoporphyrin ferrochelatase(hemH), heme oxygenase 1(HMOX1) and ferredoxin oxidoreductase(HY2), were higher than those in the green group. However, no significant differences were found between two groups on the key genes for chlorophyll synthesis including glutamyl-tRNA reductase(hemA) and magnesium chelatase(chlH, chlL, chlD). The contents of phycobiliprotein-related metabolites in the red-yellow group were higher than the green group, while the contents of the chlorophyll-related metabolism were lower than the green group. These results indicate that differentially expressed key genes for phycobiliprotein synthesis in Pyropia haitanensis resulted in difference between two groups on contents of phycoerythrin and chlorophyll a, which ultimately caused the color difference between different strains.更多还原