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The differentiation and maintenance of RORγt+ Tregs respectively require integrin β8 expression by dendritic cells and commensal microbiota

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【作者】 陈正涛王美祥刘位秀陈君兰王丁丁宋文刚唐华

【机构】 泰山医学院复旦大学附属公共卫生临床中心武汉大学

【摘要】 Backgroud: In early life, Change or absence of the symbiotic microbiota can lead to exacerbated type 2 immunity and allergic inflammations. Recent researches demonstrate that RORγt+ regulatory T cells(Tregs) regulate type 2 immune respond. The generation of it is dependent on dendritic cells and microbiota, but mechanisms are poorly understood. Methods: BM recipients were irradiated with two doses of 550 rads 3 h apart, and received 2 × 106 Zbtb46-DTR BMcells by intravenous injection.Results: DC lack of intgrin β8 resulted in loss of RORγt+ Tregs in the SI lamina propria(SILP). Loss of DCs in SILP of z DC-DTR bone marrow chimeras after treated with DT for 7 days did not influence the generation of RORγ+ Tregs. The frequencies of RORγt+ and Ki67+ RORγt+ pTregs were decreased in SILP of C57 mice after treated with antibiotics for 7 days and increased after remove antibiotics for 7 days. Oral SCFA in water help to improve the proliferation of RORγt+ p Treg subsets in SILP after treated with antibiotics. Conclusion: Integrin β8 expressed in SILP DCs play a critical role in the induction and microbiota in the gut act as a key factor on maintenance of RORγt+ pTregs.

【Abstract】 Backgroud: In early life, Change or absence of the symbiotic microbiota can lead to exacerbated type 2 immunity and allergic inflammations. Recent researches demonstrate that RORγt+ regulatory T cells(Tregs) regulate type 2 immune respond. The generation of it is dependent on dendritic cells and microbiota, but mechanisms are poorly understood. Methods: BM recipients were irradiated with two doses of 550 rads 3 h apart, and received 2 × 106 Zbtb46-DTR BMcells by intravenous injection.Results: DC lack of intgrin β8 resulted in loss of RORγt+ Tregs in the SI lamina propria(SILP). Loss of DCs in SILP of z DC-DTR bone marrow chimeras after treated with DT for 7 days did not influence the generation of RORγ+ Tregs. The frequencies of RORγt+ and Ki67+ RORγt+ pTregs were decreased in SILP of C57 mice after treated with antibiotics for 7 days and increased after remove antibiotics for 7 days. Oral SCFA in water help to improve the proliferation of RORγt+ p Treg subsets in SILP after treated with antibiotics. Conclusion: Integrin β8 expressed in SILP DCs play a critical role in the induction and microbiota in the gut act as a key factor on maintenance of RORγt+ pTregs.

【Key words】 RORγt+ Tregdendritic cellsmicrobiota
  • 【会议录名称】 第十三届全国免疫学学术大会壁报交流集
  • 【会议名称】第十三届全国免疫学学术大会
  • 【会议时间】2018-11-07
  • 【会议地点】中国上海
  • 【分类号】R392
  • 【主办单位】中国免疫学会
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