【作者】 王丽娜； 仉红； 牛卫东；

【机构】 大连医科大学口腔医学院；

【摘要】 目的:探讨Btk-PLCγ2-NFATc1信号通路在根尖周炎骨破坏中的作用机制。方法:采用双侧下颌第一磨牙开髓术后将髓腔暴露于口腔环境的方法建立大鼠根尖周炎动物模型。分别于开髓后0、1、2、3和4周各随机处死5只大鼠,分离下颌骨。制作冰冻切片后用苏木精-伊红染色方法观察大鼠根尖组织炎症情况;免疫组织化学染色检测Btk、PLCγ2、NFATc1的表达与分布情况;酶组织化学染色检测根尖组织炎症区域破骨细胞的表达,检测NFATc1的表达与破骨细胞表达的相关性。结果:(1)HE染色的结果显示,成功的构建了大鼠根尖周炎动物模型。(2)Btk、PLCγ2、NFATc1的表达随着炎症进展过程的不同而有明显的变化,在2周炎症进展的急性期,所有因子的表达均至最高值(p<0.05)。破骨细胞的数量亦是在2周时达到高峰。(3)相关性分析结果显示,Btk与PLCγ2、PLCγ2与NFATc1的表达呈高度相关,破骨细胞阳性细胞数与NFATc1的表达呈高度相关性。结论:Btk、PLCγ2、NFATc1在大鼠根尖周炎性组织中的表达量随着炎性浸润范围的扩大而增加,且与破骨细胞的表达量有显著相关性,揭示其可能与根尖周炎症反应和骨吸收作用密切相关。更多还原

【Abstract】 Objective:To explore the mechanism of Btk-PLCγ2-NFATc1 signaling pathway in bone destruction of apical periodontitis.Methods:An animal model of periapical periodontitis was established by exposing the medullary cavity to the oral environment after open the bilateral mandibular first molar.5 rats were randomly sacrificed at 0,1,2,3 and 4 weeks after opening,and the mandible bone was isolated.The frozen section was made and the inflammation of the root tip tissue was observed by hematoxylin-eosin staining.The expression and distribution of Btk,PLCγ2 and NFATc1 were detected by immunohistochemical staining.The numbers of osteoclasts of the apical tissue was detected by enzyme histochemical staining.Detecting the correlation between NFATc1 expression and osteoclasts.Results:(1) The results of FIE staining showed that the rat model of periapical periodontitis was successfully constructed.(2) The expression of Btk,PLCγ2 and NFATc1 changed significantly with the progress of inflammation.In the acute phase of inflammation progression at 2 weeks,the expression of all factors reached the highest value(p<0.05).The number of osteoclasts also peaked at 2 weeks.(3) Correlation analysis showed that the expression of Btk and PLCγ2,PLCγ2 and NFATc1 were highly correlated,and the number of osteoclast positive cells was highly correlated with the expression of NFATc1.Conclusion:The expression of Btk,PLCγ2 and NFATc1 in the periapical inflammatory tissues of rats increased with the expansion of inflammatory infiltration,and it was significantly correlated with the expression of osteoclasts,revealing that it may be associated with periapical inflammation and bone resorption.更多还原