【作者】 姜觅； 王恒彦； 汪国权；

【Author】 JIANG Mi;WANG Heng-yan;WANG Guo-quan;Shanghai Municipal Center for Disease Control and Prevention;School of Life Sciences,Shanghai University;

【机构】 上海市疾病预防控制中心； 上海大学生命科学学院；

【摘要】 建立了饮料、酒类中20种分散性染料的液相色谱测定方法。对色谱柱的种类、流动相组成、检测波长等条件进行了研究,优化了HPLC分离测定条件。样品经二氯甲烷和乙醚混合溶液提取后,采用C₁₈柱进行分离,10 mmol/L乙酸铵水溶液为流动相;20种分散性染料在75 min内达到分离。在优化条件下,20种染料在0.5～5.0mg/L范围内具有良好的线性关系（r>0.999）。在空白饮料、葡萄酒中分别按0.1、0.2、0.4mg/kg水平进行加标回收实验,其平均回收率为80%～99%,相对标准偏差为0.1%～9.1%。结果表明该方法操作简便,分析周期短,准确度较高,色谱分离效果好,适用于饮料、酒类中分散性染料的检测。更多还原

【Abstract】 A liquid chromatographic method was established for the determination of 20 disperse dyes in drink and wine. The chromatographic conditions, including analysis column types, composition of mobile phase, detection wavelengths, were optimized. The sample was extracted with dichloromethane-ethyl ether mixed solution, and the separation of 20 dyes was performed on a C₁₈ column, by using solution of 10 mmol/L ammonium acetate as mobile phase, at detection wavelengths of 430,570,640 nm. Under the optimal conditions, the calibration curves were liner in the range of 0. 5-5. 0 mg/L with correlation coefficients higher than 0. 999. The recoveries of disperse dyes from drink and wine at spiked levels of 0. 1, 0. 2, 0. 4 mg/kg were in the range of 80%-99% with RSDs of0. 1-9. 1%. The result showed that the method was simple, rapid and accurate, and was suitable for the determination of disperse dyes in drink and wine.更多还原