节点文献
大花三色堇及角堇倍性鉴定
Ploidy Identification of Viola × wittrockiana and V.cornuta
【作者】 王梦叶; 杜晓华; 贾文庆; 穆金艳; 朱小佩; 刘会超;
【Author】 WANG Meng-ye;DU Xiao-hua;JIA Wen-qing;MU Jin-yan;ZHU Xiao-pei;LIU Hui-chao;School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology;
【机构】 河南科技学院园艺园林学院;
【摘要】 为建立快速、准确的大花三色堇与角堇及其杂交后代的倍性鉴定方法,本文以11份角堇和大花三色堇自交系及其杂交产生的30份F1代为试材,采用流式细胞术法对其进行了倍性鉴定,并采用染色体压片法对检测结果进行了抽样验证。结果表明,将角堇作为流式细胞术的对照,可基本判断大花三色堇及其杂交后代的倍性,为大花三色堇杂交育种和倍性育种提供参考。
【Abstract】 In order to develop an effective method for polyploids identification in V.× wittrockiana,Viola comuta and F1 hybrids,the eleven kinds of V.× wittrockiana,Viola comuta and thirty kinds of F1 were used as experiment materials,and ploidy was measured by flow cytometry,which further was verified by squashing technique for chromosome.The results showed that the ploidy of V.× wittrockiana and its hybrids were basically determined by flow cytometry,compared with Viola comuta.This study will provide reference for the hybridization and polyploid breeding of pansy.
- 【会议录名称】 中国观赏园艺研究进展2018
- 【会议名称】2018年中国观赏园艺学术研讨会
- 【会议时间】2018-07-25
- 【会议地点】中国黑龙江哈尔滨
- 【分类号】S682.19
- 【主办单位】中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心