【作者】 范启兰； 李加林； 许春鹃； 吴素珍；

【Author】 Qilan Fan;Jialin Li;Chunjuan Xu;Suzhen Wu;Gannan Medical University;

【机构】 赣南医学院；

【摘要】 目的研究地黄多糖(Rehmannia glutinosa polysaccharides,RPS)对K562细胞增殖与凋亡的影响。方法采用CCK-8比色法检测RPS对K562细胞增殖的抑制作用,流式细胞术检测RPS对K562细胞周期及凋亡的影响,Western Blotting方法检测RPS对K562细胞中Bcl-2、Caspase-3蛋白表达的影响,采用Elisa的方法检测磷酸化的蛋白酪氨酸激酶(P-PTK)。结果用50μg/mL、100μg/mL、200μg/mL RPS处理K562细胞,48小时后,K562细胞密度明显低于对照组(P<0.05),RPS处理后的K562细胞增殖抑制率随RPS浓度增加而增加(P<0.05),其中200μg/mL RPS作用48 h后,G0/G1期细胞的比例达43.17%,显著高于对照组35.52%(P<0.05),细胞凋亡率为15.46%,也明显大于对照组(P<0.05);50μg/mL、100μg/mL、200μg/mL RPS干预K562细胞48 h后,与对照组比细胞内抗凋亡蛋白Bcl-2的表达下调(P<0.05),Caspase-3的蛋白表达升高(P<0.05);200μg/mL RPS刺激K562细胞48 h后,细胞中蛋白酪氨酸激酶的磷酸化(p-PTK)水平与对照组比较显著下降(P<0.05)。结论 RPS可抑制K562细胞增殖,使细胞增殖周期受阻;可通过调节凋亡相关蛋白Bcl-2、Caspase-3的表达以及蛋白酪氨酸激酶的磷酸化(P-PTK)水平促进K562细胞凋亡。更多还原

【Abstract】 Aim:To investigate the effect of Rehmannia glutinosa polysaccharides on the proliferation and apoptosis of K562 cells.Methods:The proliferation of K562 cells inhibited by RPS was measured by CCK-8 colorimetric method and the apoptosis and cell cycle was detected by flow cytometry.The protein expressions of Bcl-2 and Caspase-3 were detected by Western blot.The phosphorylated protein tyrosine kinase was detected by Elisa.Results:After treated K562 cells with 50 μg/mL,100 μg/mL and 200 μg/mL RPS for 48 hours,the density of K562 cells was significantly lower than that of the control(P<0.05).The inhibitory rate of the cellular growth of K562 cells increased gradually by increasing RPS concentration(P<0.05).The ratio of cells at G0/G1 phase was 43.17%after treatment with 200 μg/mL RPS for 48 h,which was significantly higher than that in the control group and the apoptotic rate was 15.46%,which was significantly higher than that of the control group(P <0.05).The expression of Bcl-2 of k562 cells treated by 50,100,200 μg/mL RPS for 48 h were lower than that of the control(P<0.05),and the expression of Caspase-3 was higher than that of the control(P<0.05).Compared with the control group,the P-PTK was decreased after incubated the K562 cells with RPS for 48 h(P<0.05).Conclusion:RPS could block the cell cycle in G0/G1 phases and then inhibited the proliferation of K562 cells.RPS could promote the apoptosis of K562 cells by regulating the expression of Bcl-2,Caspase-3 and phosphorylation of protein tyrosine kinase(P-PTK) levels.更多还原