【作者】 魏盛； 肖新洋； 张浩； 李亚琼； 高杰； 朱德豪； 乔明琦；

【Author】 Sheng Wei;Xinyang Xiao;Hao Zhang;Yaqiong Li;Jie Gao;Dehao Zhu;Mingqi Qiao;Department of Neurosurgery,Qilu Hospital of Shandong University and Brain Science Research Institute,Shandong University;Lab of Traditional Chinese Medicine Classical Theoiy,Ministiy of Education,Shandong University of Traditional Chinese Medicine;Lab of Behavioral Brain Analysis,Shandong University of Traditional Chinese Medicine;

【机构】 山东大学齐鲁医院脑科学研究所； 山东中医药大学中医药经典理论教育部重点实验室； 山东中医药大学脑行为分析实验室；

【摘要】 目的已有研究表明母代慢性应激或抑郁与子代增加的情感障碍风险有关,然而,动物实验上有关母代孕前慢性应激对子代行为究竟有何影响仍不清楚,通过母鼠孕前怒情绪应激来探讨其对雄性子代的影响。方法将孕前母鼠暴露于21天居住入侵应激(怒情绪应激),随后考察其成年雄性子代行为表征及其神经生物机制。怒情绪应激采用居住入侵范式并用攻击行为测试来评价。结果怒情绪应激母鼠运动量显著增加并富于攻击,糖水偏好则显著减少。暴露于孕前怒情绪应激下的雄性子代则显示出增强的攻击行为,焦虑样行为和糖水偏好均显著减少;认知方面,暴露于孕前怒情绪应激下的雄性子代显示物体识别实验中分辨指数及Morris水迷宫中穿越平台次数显著降低,提示有明显认知缺损。暴露于孕前应激条件下雄性子代并有部分脑区单胺能系统及BDNF,P-CREB和SERT蛋白表达的显著改变。对暴露于孕前应激条件下母代预先予氟西汀干预可显著减轻雄性子代的部分行为及神经生化指标异常。结论孕前怒情绪应激可诱导子代攻击行为,认知缺损及相应神经生化改变。更多还原

【Abstract】 Purpose Studies have shown that maternal chronic stress or depression is linked to an increased risk for affective disorders in progeny.However,the impact of maternal chronic stress before pregnancy on their progeny in animal models has not been well studied.MethodsWe investigated the behaviors and the neurobiology in 60-day-old male progeny of maternal rats exposed to a 21-day resident intruder stress before pregnancy.Anger stressed parental rat model was established using the resident-intruder paradigm and evaluated by behavioral tests.ResultsAnger stressed maternal rats showed a significant increasing in locomotion and aggressivity but a reduction in sucrose preference.Offspring subjected to pre-gestational anger stress displayed enhanced aggressive behaviors,reduced anxiety and sucrose preference.With regard to cognition,offspring subjected to pre-gestational stress showed significant impairments in recognition index(RI) of the object recognition test and number of platform crossing in Morris water maze test.The monoaminergic system were significantly altered in pre-gestationally stressed offspring and the expression of BDNF,P-CREB,and SERT levels in pre-gestational stressed offspring were also altered in some brain regions.Fluoxetine were used to treat pre-gestational stressed maternal rats and could significantly relieve changes caused by stress in both behaviors and neural biochemical indexes of offspring,but not in all cases.Conclusion Anger stress before pregnancy could induce aggressive behaviors,cognitive deficits,and neurobiological alterations in the offspring.更多还原