【作者】 刘德祥； 吴惠然； 潘芳；

【Author】 Liu de-xiang;Wu huiran;Pan Fang;Department of Medical Psychology and Ethics, School of Basic Medical Science, Shandong University;

【机构】 山东大学基础医学院医学心理学系与伦理学系；

【摘要】 早年创伤性应激对成年期大鼠焦虑和抑郁行为、前额叶及海马5-HT1A受体、5-HT2C受体表达的影响,自主运动对上述指标的改善作用。1.健康雄性青少年期(21日龄)Wistar大鼠33只,随机分为:对照组、PTSD组和运动组。以不可逃避的足底电击(6天)建立PTSD动物模型,以规律自主运动作为干预手段。以高架十字迷宫和旷场试验检测大鼠焦虑和抑郁行为。采用免疫组织化学法和免疫印迹法检测前额叶和海马5-HT受体亚型表达。与对照组相比,PTSD大鼠在旷场试验和高架十字迷宫试验中表现出更明显的抑郁和焦虑样行为。PTSD大鼠前额叶5-HT1AR表达升高,前额叶和海马5-HT2CR表达增加。规律自主运动可明显改善焦虑和抑郁样行为,降低PTSD大鼠前额叶5-HT1AR、前额叶和海马5-HT2CR的表达水平。早年创伤性应激可导致大鼠产生持续至成年的焦虑与抑郁样行为、前额叶5-HT1AR、5-HT2CR以及海马5-HT2CR表达改变。提示5-HT受体功能异常与PTSD病理机制相关。更多还原

【Abstract】 The aim of the study is to observe the dynamitic changes of depressive and anxiety like behaviors, 5-HT receptors expression in PFC and HIP in adolescent rats and adult rats which experienced adolescent traumatic stress; and the effects of progressive treadmill exercise, paroxetine hydrochloride and CRF1 receptor antagonist(CP-154, 526) on the depressive and anxiety like behaviors of rats; and also 5-HT receptors expression in prefrontal cortex and Hippocampus. 33 rats(male, 21 days old) were randomly distributed into three groups(n=11 per group): the control group, the PTSD group, the exercise group. The PTSD animal model was established by inescapable electric foot shock stress. The behavioral changes were assessed by the elevated plus maze test and the open field test. Immunohistochemistry test and Western Blotting test were used to calculate the expression of 5-HT receptors in PFC and HIP. Compared with control group, PTSD rats showed anxiety and depression-like behaviors and a significant enhancing expression of 5-HT1 AR in PFC, 5-HT2 CR in PFC and HIP; while regular treadmill exercise improved anxiety and depression-like behaviors, decreased the 5-HT1 AR expression in PFC, 5-HT2 CR in PFC and HIP in exercise group. Early-life traumatic stress resulted in a persisted anxiety and depression-like behaviors in both adolescence and adult rats; led to dysfunctions of 5-HT1 AR in PFC and 5-HT2 CR in both PFC and HIP.更多还原