Role of mating-type genes in sexual reproduction and pathogenicity of Magnaporthe oryzae

【作者】 李玲； GuoXiaoyu； Wang Yanli； 王教瑜； 孙国昌；

【Author】 Li Ling;GuoXiaoyu;Wang Yanli;Wang Jiaoyu;Sun Guochang;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences;The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,School of Agricultural and Food Sciences,Zhejiang Agriculture and Forest University;

【机构】 State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences； The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,School of Agricultural and Food Sciences,Zhejiang Agriculture and Forest University；

【摘要】 Magnaporthe oryzae is the causal agent of rice blast disease,the most devastating of cultivated rice(Oryza sativa) and a continuing threat to global food security.M.oryzae,as a heterothallic fungus,also produces sexual generation,but needs two parent strains carrying opposite mating-types to complete the mating process.M.oryzae has two mating types of species,MAT1-1 and MAT1-2,The MAT1-1 gene locuscontains three genes,MAT1-1-1,MAT1-1-2 and MAT1-1-3,and the MAT1-2 locus contains two genes,MAT1-2-1 and MAT1-2-2.In the present work,we investigated the roles of the five mating genes,in sexual reproduction and pathogenicity of M.oryzae.Via homologous recombination,MAT1-1-1,MAT1-1-2 and MAT1-1-3 were respectively deleted from a M.oryzae MAT1-1 strain,and MATl-2-1 and MAT1-2-2 were deleted from a M.oryzae MAT1-2 strain.The phenotypical analysis indicated that MAT1-1-1 and MAT1-1-3 were involved in vegetative growth,MAT1-1-1,MAT1-1-2,MAT1-1-3 participated in sporulation,and MAT1-2-2 mutant was likely affected in cell wall integrality.Crossing-cultured with a strain in opposite mating type,△mat1-1-1,△mat1-1-2,△mat1-1-3 and △mat1-2-1,other than △mat1-2-2,formed much fewer perithecia,suggesting that the MAT1-1-1,MAT1-1-2,MAT1-1-3 and MAT1-2-2 are required for sexual reproduction in M.oryzae.However,inoculation test showed that the pathogenicity was unaltered in these mutants.更多还原

【Abstract】 Magnaporthe oryzae is the causal agent of rice blast disease,the most devastating of cultivated rice(Oryza sativa) and a continuing threat to global food security.M.oryzae,as a heterothallic fungus,also produces sexual generation,but needs two parent strains carrying opposite mating-types to complete the mating process.M.oryzae has two mating types of species,MAT1-1 and MAT1-2,The MAT1-1 gene locuscontains three genes,MAT1-1-1,MAT1-1-2 and MAT1-1-3,and the MAT1-2 locus contains two genes,MAT1-2-1 and MAT1-2-2.In the present work,we investigated the roles of the five mating genes,in sexual reproduction and pathogenicity of M.oryzae.Via homologous recombination,MAT1-1-1,MAT1-1-2 and MAT1-1-3 were respectively deleted from a M.oryzae MAT1-1 strain,and MATl-2-1 and MAT1-2-2 were deleted from a M.oryzae MAT1-2 strain.The phenotypical analysis indicated that MAT1-1-1 and MAT1-1-3 were involved in vegetative growth,MAT1-1-1,MAT1-1-2,MAT1-1-3 participated in sporulation,and MAT1-2-2 mutant was likely affected in cell wall integrality.Crossing-cultured with a strain in opposite mating type,△mat1-1-1,△mat1-1-2,△mat1-1-3 and △mat1-2-1,other than △mat1-2-2,formed much fewer perithecia,suggesting that the MAT1-1-1,MAT1-1-2,MAT1-1-3 and MAT1-2-2 are required for sexual reproduction in M.oryzae.However,inoculation test showed that the pathogenicity was unaltered in these mutants.更多还原