【作者】 徐海燕； 李敏； 王宇； 沈玲玲； 孙志宏； 张和平；

【Author】 Haiyan Xu;Min Li;Yu wang;Lingling Shen;Zhihong Sun;Heping Zhang;Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education P.R.C.,Key Laboratory of Dairy Products Processing,Ministry of Agriculture P.R.C.,Inner Mongolia Agricultural University;

【机构】 内蒙古农业大学乳品生物技术与工程教育部重点实验室农业部奶制品加工重点实验室；

【摘要】 益生菌已经被证实能够促进动物生长并维持动物的健康。然而,较少的研究关注益生菌对宠物健康的影响,我们选择了90只不同年龄的犬作为研究对象,根据犬的年龄分成3组(老年组,n=30;年轻组,n=24;和培训组,n=36)。每组平均分为试验组和对照组,试验组每天将复合益生菌制剂(Lactobacillus casei Zhang, Lactobacillus plantarum P-8,和Bifdobacterium animalis subspecies lactis V9)作为饲料添加剂饲喂60天。分别在第0天、30天、60天和饲喂停止的15天后采集所有犬的血液和粪便样品进行研究。结果表明,益生菌有促进犬肠道菌群维持稳态的作用,尤其是饲喂益生菌的老年犬的肠道菌群较对照组更稳定。有趣的是,在益生菌饲喂第30天时,老年犬(而不是幼犬和训练犬)的平均每日进食量(ADFI)显著增加(P<0.05)。在益生菌饲喂30到60天期间,所有犬的平均每日体重长量均显著增加(P<0.05);然而,只有在老年组中,这种效果持续到停止益生菌饲喂15天后。所有的犬,益生菌的添加均使得一些有益细菌显著增长(如乳杆菌属的一些菌种和柔嫩梭菌),潜在的有害细菌显著降低(如Fusobacterium perfoetens和Sutterella stercoricanisin)。益生菌的应用也增强了犬的免疫力,表现为益生菌组的血清IgG(P<0.001)和粪便SIgA(P<0.001)的水平显著高于对照组。该研究结果为益生菌在宠物中的应用提供了新的数据,并为基于微生态改善动物健康的发展奠定基础。更多还原

【Abstract】 Probiotics have been used successfully to promote growth and maintain animal health. However, only limited studies have focused on the health-enhancing effects of probiotics in dogs, particularly on the age-specific effect. A total of 90 dogs were divided into 3 groups based on dog age(elderly group, n=30; young group, n=24; and training group, n=36). Each group was subdivided into 2 subgroups, with and without receiving daily treatment of compound probiotics(containing the strains Lactobacillus casei Zhang, Lactobacillus plantarum P-8, and Bifdobacterium animalis subspecies lactis V9) as feed additive. Serum and fecal samples were collected at 4 different time points, i.e. day 0, 30, 60, and 15 days after ceasing probiotics treatment. Our results showed that probiotics application improved the stability of canine gut microbiota, especially in elderly dogs. It was interesting to observe that only the average daily feed intake(ADFI) of the elderly dogs(but not the young and training group) significantly increased after 30 days of probiotics administration(P<0.05), although the overall ADFI of the elderly dogs were still lower than that of the other groups. The average daily weight gain of all groups significantly increased between day 30 and day 60(P<0.05); however, only in the elderly group, the effect lasted until 15 days after ceasing probiotics treatment. For all dog groups, the probiotics application stimulated the growth of some beneficial bacteria(including some Lactobacillus species and Faecalibacterium prausnitzii) and inhibited certain potentially harmful ones(including Fusobacterium perfoetens and Sutterella stercoricanisin). The probiotics application also enhanced canine immunity, as shown by the higher serum IgG(P<0.001) and fecal SIgA(P<0.001) levels in the probiotics groups compared with the controls in most cases. The current findings have provided interesting insights into the application of probiotics in pet animals and future development of probiotic-based strategies to improve animal health.更多还原