【作者】 王莉； 王刚； 尚丛娣； 房喻；

【Author】 Li Wang;Gang Wang;Congdi Shang;Yu Fang;Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Shaanxi Normal University;

【机构】 应用表面与胶体化学教育部重点实验室,陕西师范大学；

【摘要】 设计合成了水溶性小分子萘二甲酰亚胺荧光探针。研究发现,该探针的荧光性质及荧光各向异性强烈依赖于介质极性和粘度。同时,该探针在水溶液中显示出较高的荧光量子产率(～20%)和较长的荧光寿命(～3.7ns)。进一步研究表明,该探针可以富集在阴离子聚集体表面,从而实现对阴离子表面活性剂聚集的选择性检测。受此启发,将该探针置于表面带负电荷的生物膜体系中,可实现对细胞膜和大肠杆菌的监测,由此有可能实现对水体微生物污染的快速检测。更加重要的是,所有检测均可通过可视化的方式实现。基于此,该荧光分子有望应用于化学传感及生物化学成像。更多还原

【Abstract】 A naphthalimide-based low-molecular mass fluorophore(NA) was designed and synthesized. UV-vis absorption and fluorescence measurements revealed that the fluorophore is soluble in water, and shows a fluorescent quantum yield of ～20% and lifetime of ～3.7 ns. Moreover, the fluorescence emission and anisotropy of the fluorophore as produced are both dependent upon the viscosity and polarity of the medium. Further studies demonstrated that NA can be used as a selective probe to monitor the aggregation of anionic surfactants owing to its accumulation onto the anionic surfaces of the aggregates as formed. Inspired by the discovery, NA was successfully applied for detection of cell membranes and E. Coli via monitoring their negatively charged surfaces, which is important for fast checking of biological contamination of water. Importantly, all the tests could be performed in a visualized manner. We believe that the new, low molecular mass fluorophore as created may find applications in chemical and biochemical sensing and imaging.更多还原