【作者】 付礼胜； 侯宁； 王晓燕； 高雪丽； 刘超男； 吕晓萍； 郑世民；

【Author】 FU Li-sheng;HOU Ning;WANG Xiao-yan;GAO Xue-li;LIU Chao-nan;LV Xiao-ping;ZHENG Shi-min;Colloge of Veterinary Medicine,Northeast Agricultural University;Qinhuangdao Saintland Ocean Park;

【机构】 东北农业大学动物医学学院黑龙江省实验动物与比较医学重点实验室； 秦皇岛圣蓝皇家海洋公园；

【摘要】 为探索禽网状内皮组织增生病病毒(REV)感染对雏鸡中枢免疫器官细胞凋亡及其相关酶活性和基因表达的影响。将70只1日龄SPF雏鸡随机分为REV感染组和未感染病毒的对照组,应用酶活性检测、荧光定量PCR、ELISA、TUNEL等方法对胸腺和法氏囊细胞的Caspase-3和Caspase-9活性、Bcl-2 m RNA及其蛋白表达和凋亡细胞的动态变化进行了系统检测。结果发现,REV感染SPF雏鸡后,其上述免疫器官无论是细胞凋亡数量还是Caspase-3与Caspase-9活性、Bcl-2 m RNA及其蛋白表达均不同程度的高于未感染病毒的对照雏鸡。表明REV感染引起SPF雏鸡中枢免疫器官Caspase-3和Caspase-9活性增强、Bcl-2 m RNA及其蛋白表达上调所致的细胞凋亡与雏鸡免疫器官功能抑制密切相关。更多还原

【Abstract】 In order to explore the effects of cells apoptosis,enzyme activity and expression of relevant factor in central immune organs of chicks infected with Reticuloendotheliosis virus.70 one day old SPF chicks were randomly divided into REV infected groups and blank control groups.The dynamic changes of Caspase-3 and Caspase-9 activity,cells apoptosis,Bcl-2 m RNA and protein expression in central immune organs were detected by enzyme activity detection kit,TUNEL,RT-PCR,ELISA.The results showed that abovedetected indexes in central immune organsof SPF chicks infected with REV were varying degrees higher than blank control groups.These data indicate that cell apoptosis caused by enhancement of Caspase-3 and Caspase-9 activity,up-regulated expression of the Bcl-2 m RNA and protein in central immune organs of SPF chicks infected with REV,are closely related to immune function suppression.更多还原