【作者】 武和英； 王秀华； 杨冰； 于党辉； 黄倢；

【Author】 WU Heying;WANG Xiuhua;YANG Bing;HUANG Jie;YU Danghui;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;

【机构】 上海海洋大学水产与生命学院； 中国水产科学研究院黄海水产研究所农业部海洋渔业可持续发展重点实验室；

【摘要】 从罗非鱼（Oreochromis Niloticus）循环水养殖系统中分离到一株具有高效脱氮特性的菌株（编号DZYC02）,分别用葡萄糖、蔗糖、可溶性淀粉、丁二酸钠、乙酸钠、柠檬酸钠为碳源,研究了碳源种类对菌株DZYC02脱氨氮效果的影响;以蔗糖为碳源、NH₄Cl为氮源,研究了不同碳氮比、初始pH及盐度对该菌株脱氮效果的影响。结果表明,菌株DZYC02在以柠檬酸钠为碳源、C:N≥15、p H 5⁷、盐度0¹5的条件下具有良好的脱氮效果,24 h内对浓度为20 mg/L的NH4+的去除率达100%,48h内对浓度为20 mg/L的NO²—的去除率高达100%;将该菌采用浸泡方式感染斑马鱼（Danio rerio）进行生物安全试验,结果表明菌株DZYC02对斑马鱼表现出较好的安全特性。分别用biolog细菌鉴定方法和16S r DNA序列分析比对法对该菌进行鉴定,结果显示菌株DZYC02为一株肺炎克雷伯氏菌（Klebsiella.pneumoniae）。本研究结果可为循环水养殖中开发利用微生物脱氮技术提供理论支持更多还原

【Abstract】 A bacterial strain with denitrification activity was isolated from a tilapia（Oreochromis Niloticus） recirculating aquaculture system and designated as DZYC02. Glucose, sucrose, soluble starch, sodium succinate, sodium acetate and sodium citrate were used to determine the effects of carbon source on the denitrifying efficacy of DZYC02 strain. Also sodium citrate and ammonia were used as carbon source and nitrogen source to determine the effects of C: N, p H and salinity on the denitrifying efficacy. The results showed that the 24h-denitrifying rate was 100% for ammonia（NH₄⁺-N） and 48h-denitrifying rate was 100% for nitrite（NO₂^—-N） under the condition of sodium citrate being used as the carbon source, C:N ≥15, initial p H5⁷ and salinity between 0¹5. Zebra fish（Danio rerio） were used to evaluate the biosafety of strain DZYC02 by immersion challenge, and the results showed that strain DZYC02 appear better safety features for fish. Bacterial identification by both biolog microbes identification system and 16 S r DNA sequencing show that strain DZYC02 was a Klebsiella pneumoniae. The results of this study could provide theoretical support for the utilization of microbial denitrification technology in recirculating aquaculture system.更多还原