【作者】 陈素华； 李菲； 乔慧； 傅洪拓； 孙盛明； 张文宜； 金舒博； 龚永生； 蒋速飞； 熊怡伟； 吴滟；

【Author】 CHEN Suhua;LI Fei;QIAO Hui;FU Hongtuo;SUN Shengming;ZHANG Wenyi;JIN Shubo;GONG Yongsheng;JIANG Sufei;XIONG Yiwei;WU Yan;Wuxi Fisheries College, Nanjing Agricultural University;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences;

【机构】 南京农业大学无锡渔业学院； 中国水产科学研究院淡水渔业研究中心农业部淡水渔业和种质资源利用重点实验室；

【摘要】 本研究在青虾中分离和鉴定了两个生物钟基因period(Mnper)和timeless(Mntim)。Mnper基因cDNA全长4283 bp,编码1292个氨基酸,包括一个Per-Arnt-Sim(PAS)结构域,一个胞质定位域(CLD),一个TIM蛋白结合位点(TIS),和一个核定位信号(NLS)。Mntim基因编码1540个氨基酸,包括一个PER蛋白结合位点(PIS),一个NLS,和一个CLD。雌雄成体组织表达表明,两基因在眼柄和脑中高表达,同时在卵巢中也有表达。模拟繁殖季节和非繁殖期表达模式研究发现,Mnper和Mntim在眼柄中的表达不受外界环境因子的影响,但在脑中的表达表现出显著的季节性变化。因此,我们推测脑是这两个生物钟基因主要的功能部位。不同的模拟季节和卵巢不同发育时期中的表达模式结果显示,这两个基因可能与雌性生殖有关。更多还原

【Abstract】 In this study, we isolated and characterized two circadian clock genes in Macrobrachium nipponense, period(Mnper) and timeless(Mntim). The complete Mnper cDNA measures 4283 bp in length with an open reading frame encoding 1292 amino acids, including functional domains such as PER-ARNT-SIM(PAS), cytoplasmic localization domain(CLD), TIM interaction site(TIS), and nuclear localization signal(NLS). The deduced Mntim protein comprises1540 amino acids with functional domains such as PER interaction site(PIS), NLS, and CLD. Tissue distribution analyses showed that the two genes were highly expressed in the eyestalk and brain in both males and females, as well as being expressed in the ovary. The expression profiles of Mnper and Mntim were determined in the eyestalk, brain, and ovary under simulated breeding season and non-breeding season conditions. The expression profiles of both Mnper and Mntim appeared to be unaffected in the eyestalk. However, the expression of both genes exhibited significant seasonal variations in the brain, and thus we assumed the brain to be their functional location. The expression profiles under different simulated seasons and the variations during different ovarian stages indicate that both genes might be involved with female reproduction.更多还原