【作者】 王川林； 梅小平； 杨正伟； 杨霞； 袁东； 彭彬；

【Author】 WANG Chuanlin;MEI Xiaoping;YANG Zhengwei;YANG Xia;YUAN Dong;PENG Bin;Department of Infectious Disease,The Affiliated Hospital of North Sichuan Medical College;Morphometric Research Laboratory,North Sichuan Medical College;Department of Clinical Medicine,North Sichuan Medical College;Research Unit of Electron Microscopy Structures,North Sichuan Medical College;

【机构】 川北医学院附属医院感染科； 川北医学院形态定量研究室； 川北医学院临床医学系； 川北医学院电镜结构研究室；

【摘要】 目的:探讨四氯化碳（CC1₄）对大鼠肝组织内胶原纤维的体积以及肝星状细胞的结构、数量的影响。方法:24只三月龄雄性SD大鼠随机分为2组:肝纤维化模型组大鼠背部皮下注射40%CCl₄混悬液（3ml/kg）,每周2次,对照组等时等量皮下注射橄榄油,每组分别于开始注射后5周和10周取材,按照等距随机抽样原则从每只大鼠肝脏抽选4个组织块制作石蜡包埋切片,行Masson染色显示胶原纤维;另从每只大鼠肝脏随机抽选5个组织块制作电镜切片。初步定性观察肝组织内肝小叶、肝细胞、肝星状细胞以及胶原纤维的体积或数量改变。结果:光镜及电镜定性观察结果显示,随着CCl₄注射时间的延长,中央静脉周围、窦周隙和门管区的胶原纤维逐渐增多,并分割正常肝小叶形成假小叶;窦周隙内肝星状细胞数量逐渐增加,胞质内脂滴减少,粗面内质网与高尔基体显著增加。结论:CCl₄诱导大鼠肝组织内胶原纤维增加、假小叶形成,可能与肝星状细胞的活化及数量增加有关。此结论还需要我们后续进行体视学定量研究来证实。更多还原

【Abstract】 Objective:To investigate the effect of carbon tetrachloride（CC1₄） on the volume of collagenous fibers and the structure and number of hepatic stellate cells（HSCs） in the rat liver.Methods:24 rats were randomly divided into 2 groups:liver fibrosis model group were injected subcutaneously with40%CC1₄ suspension（3 ml/kg） and the control group were injected with equivalent olive oil,2 times a week.5 and 10 weeks after the initial injection,4 tissue blocks were sampled in a systematic（equally spaced） manner and were embedded in paraffin.Then,paraffin-embedded sections were prepared and stained with Masson trichrome staining to show the collagenous fibers.In addition,5 liver tissue blocks were also sampled randomly from each rat and electron microscopic sections.The changes in the volume or number of hepatic lobules,hepatocytes,HSCs and collagenous fibers in the rat liver were preliminary qualitative observed.Results:The results of qualitative observation of light and electron microscopy showed that:with the development of liver fibrosis,the collagenous fibers in the perisinusoidal space and portal or around the central vein increased gradually,and eventually divided normal hepatic lobules into pseudolobule;the number of HSCs in the perisinusoidal space significantly increased.Conclusion:The increase in the volume of collagenous fibers and the formation of pseudolobule in the rat liver tissue,induced by the injection of CC1₄,part of the reason may be associated with the activation and prolifration of HSCs,although this conclusion needs to be confirmed by continued stereological quantitative research we would conducted.更多还原