【作者】 杜倩； 乌兰； 刘睿； 樊蕊； 陈启贺； 任金威； 李勇；

【机构】 北京大学医学部公共卫生学院营养与食品卫生学系；

【摘要】 【目的】探讨核桃肽(WP)对幼年小鼠学习记忆能力的改善作用及其机制。【方法】选用48只初断乳C57BL/6J雄性小鼠,随机分成4组,空白对照组和低、中、高三个WP组(110mg/kg·bw、220mg/kg·bw和440mg/kg·bw),每组12只,干预30天后进行开阔场实验、Morris水迷宫实验、跳台实验和穿梭箱实验,观察小鼠的自主活动能力及情绪反应、空间记忆能力和主动及被动回避能力。【结果】Morris水迷宫结果显示,WP高剂量组小鼠的逃避潜伏期较空白对照组明显缩短(P<0.05),WP中、高剂量组在目标象限停留时间和穿台次数较空白对照组明显增加(P<0.05);跳台实验24h测试中,与空白对照组相比,WP低、高剂量组错误次数减少(P<0.05),三个干预组停留潜伏期均增长(P<0.05),5d后测试中,WP中剂量组对电击的记忆消退较慢(P<0.05)。穿梭箱实验中,与空白对照组相比,WP低、中、高干预组小鼠的主动回避次数增多(P<0.05),受到电击次数减少(P<0.05)。核桃肽剂量组血清T-SOD及GSH-Px活性高于空白对照组,MDA水平低于空白对照组(P<0.05)。【结论】核桃肽可以增强幼年小鼠学习和空间记忆能力、主动及被动回避能力,也可能具有延缓小鼠学习记忆的衰退的作用,其机制可能与核桃肽的抗氧化性有关。更多还原

【Abstract】 【Objective】 To investigate the improvement effects and their mechanisms of walnut peptides(WP)on learning and memory abilities in young mice.【Methods】 48 male weaning C57BL/6J mice were randomly divided into 4 groups,the control group,low WP intervention group,middle WP intervention group,and high WP intervention group(the concentrations of WP were 110mg/kg·bw,220mg/kg·bw and 440mg/kg·bw respectively),with 12 mice in each group.After30 days feeding,the Open-field test,Morris Water Maze,Step-down test and Shuttle test were performed,in order to observe the ability of autonomic activities and emotional responses,spatial memory ability,and the active and passive avoidance reactions in mice.【Result】 According to the results of Morris Water maze,the escape latency of high WP group was significantlyreduced compared with the control group(P<0.05),and for middle and high WP groups,the times staying in the target quadrant and the numbers of crossing over the position where the platform had been were increased compared with the control group(P<0.05).The Step-down test indicated,compared to the control group,the low and high WP groups had less error times(P<0.05),and three intervention groups had longer step-down latency(P<0.05).After the training 5 days,the memory of shocking in the middle WP group declined more slowly(P<0.05).Shuttle test showed that,compared with the control group,after the intervention of the low,middle,and high doses of WP,the numbers of active avoidance reaction increased(P<0.05),while the times of electric shock decreased in mice(P<0.05).The activities of T-SOD and GSH-Px in three WP groups were higher,and the level of MDA were lower compared with the control group(P<0.05).【Conclusion】 The walnut peptides could enhance the abilities of learning and spatial memory and the abilities of active and passive avoidance in young mice,also they may have the effect to delay the decline in learning and memory of mice.The mechanism was related to the antioxygenic property of WP.更多还原