【作者】 雷蕾； 蒋瑾瑾；

【Author】 LEI Lei;JIANG Jin jin;Changhai Hospital Afiliated to the Second Military Medical University;

【机构】 第二军医大学附属长海医院；

【摘要】 目的:通过趋化因子研究H2R及H4R在儿童HSP发生发展过程中可能的作用机制。方法:检测HSP患者外周血单个核细胞上H2R,H4R及细胞趋化因子CXCL16、CCL17的表达,及两者之间的相关性。结果:1.急性期HSP患儿体内PBMC上H2R及H4R mRNA和蛋白的表达明显高于健康对组(P=0.00),2.急性期HSP患儿CXCL16、CCL17mRNA及蛋白水平较缓解期组以及健康对照组显著升高(P<0.01),缓解期组的表达水平较健康对照组明显升高(P<0.01),且两种因子的表达与H2R及H4R呈正相关。结论:H2R及H4R参与了HSP急性期炎症的发生发展,急性期趋化因子CXCL16和CCL17分泌增多,且与H2R及H4R的表达呈正相关性。H2R及H4R可能通过这一途径参与HSP炎症反应的发生发展。更多还原

【Abstract】 Objective:To study the role of H2 R/H4 R in the process of development and its mechanism of children HSP.Methods:Detect the expression of CXCL16,CCL17 and p-STAT1 in PBMC from patients in active phase and remission phase,and analysis their correlation with H2 R and H4 R.Result:1.The histamine receptor H2 R and H4 R of acute phase was significantly higher than that of remission and healthy children(P=0.00).2.The gene and protein expression of CXCL16,CCL17 from HSP in active period were much higher than the level of remission and healthy control groups(p<0.01),and there was a significantly positive correlation between the expression of CCL17/CXCL16 and H2 R/H4 R.Conclusion:In the acute phase of HSP,the upregulation of CXCL16 and CCL17 on PBMC can be observed,which have a positive correlation with expression of H2 R and H4 R.The H2 R and H4 R may participate in the pathogenesis of HSP through chemokine.更多还原