The Adhesion and Neurite Outgrowth of Neurons on Poly(D-lysine)/Hyaluronan Multilayer Films

【作者】 石海飞； 盛国平； 徐骥华； 陈博； 吴守成；

【机构】 浙江大学医学院附属第一医院手外科； 浙江大学医学院附属第一医院传染病诊治国家重点实验室；

【摘要】 Poly(D-lysine)/hyaluronan(PDL/HA)films were prepared using layer-by-layer technique and chemically cross-linked with a water soluble carbodiimide(EDC)in combination with N-hydroxysuccinimide(NHS)through formation of amide bonds.Quartz crystal microbalance with dissipation(QCM-D)was used to follow the cross-linking reaction.Atomic force measurement,ellipsometry,and Fourier transform infrared(FTIR)spectroscopy were performed to study the chemical structure,topology,thickness and mechanical properties of the cross-linked films.QCM-D and Frictional force study were used to reveal the viscoelasticity of the films after cross-linking treatment.The stability of the films was studied via incubating the films in physiological environment.Finally,the neurons were used to evaluate the interaction between films and cells.The results indicated that the neurons were preferably proliferating and outgrowth neurite on cross-linked films while uncross-linked films are highly cell resistant.更多还原

【Abstract】 Poly(D-lysine)/hyaluronan(PDL/HA)films were prepared using layer-by-layer technique and chemically cross-linked with a water soluble carbodiimide(EDC)in combination with N-hydroxysuccinimide(NHS)through formation of amide bonds.Quartz crystal microbalance with dissipation(QCM-D)was used to follow the cross-linking reaction.Atomic force measurement,ellipsometry,and Fourier transform infrared(FTIR)spectroscopy were performed to study the chemical structure,topology,thickness and mechanical properties of the cross-linked films.QCM-D and Frictional force study were used to reveal the viscoelasticity of the films after cross-linking treatment.The stability of the films was studied via incubating the films in physiological environment.Finally,the neurons were used to evaluate the interaction between films and cells.The results indicated that the neurons were preferably proliferating and outgrowth neurite on cross-linked films while uncross-linked films are highly cell resistant.更多还原