【作者】 孙榅淑； 王俊； 周骏； 马雁军； 白若石； 杨惠娟； 许东亚； 焦哲恒； 史宏志；

【Author】 SUN Wenshu;WANG Jun;ZHOU Jun;MA Yanjun;BAI Ruoshi;YANG Huijuan;XU Dongya;JIAO Zheheng;SHI Hongzhi;National Tobacco Cultivation & Physiology & Biochemistry Research Center, Tobacco Cultivation Key Laboratory of China Tobacco;Beijing Cigarette Factory;

【机构】 河南农业大学国家烟草栽培生理生化研究基地/烟草行业烟草栽培重点实验室； 上海烟草集团北京卷烟厂；

【摘要】 为研究硝态氮含量对调制后烟叶高温贮藏过程中TSNA形成的影响,设计白肋烟田间施氮肥量试验、室内对烤烟烟叶添加不同浓度硝酸盐试验,并对白肋烟和烤烟的叶片和烟梗分别取样,得到硝态氮含量差异较大样品。将样品在高温(45℃)条件下贮藏15 d,以在低温(10℃)条件下贮藏作为对照,测定所有样品的硝态氮和TSNA含量。结果表明:烟叶中硝态氮含量与施氮量呈正相关;新调制的烟叶硝态氮含量与TSNAs相关性较小,在高温贮藏15 d后,其相关性显著增加,烟叶硝态氮含量与高温贮藏期间TSNAs的增加量呈显著正相关;白肋烟和烤烟均表现出烟叶主脉中的硝态氮含量远高于叶片,高温贮藏15 d后,主脉TSNA增加量显著高于叶片;在人工添加硝酸盐的试验中,随添加硝酸盐量的增加,烤烟烟叶硝态氮含量及高温贮藏后TSNA含量显著增加。以上说明硝态氮含量与高温贮藏过程中TSNAs的形成有密切关系,通过农业措施降低烟叶硝态氮积累是减少贮藏过程中TSNA形成的有效途径。更多还原

【Abstract】 Field experiment was conducted to determine the effect of nitrate nitrogen content on TSNAs formation during high temperature storage. Different concentration of nitrate was applied in tobacco growing season. Leaf samples from lamina and midrib of both burley and fl ue-cured tobacco were collected. Lab experiment was conducted in a temperature-controlled chamber at 45℃ and 10℃ for 15 d. Nitrate nitrogen and TSNA contents of all samples were determined. Results indicated that nitrate nitrogen content increased with the increment of nitrogen fertilizer. Content of nitrate nitrogen and TNSA had a low correlation in newly cured tobacco. However, after high temperature storage for 15 d, the correlation signifi cantly increased. Nitrate nitrogen content and TSNAs increment during high temperature storage showed a signifi cant positive correlation. Nitrate nitrogen content in midrib was much higher than that in lamina in both burley and fl uecured tobacco. After high temperature storage for 15 d, TSNAs increment in midrib was significantly higher than that in lamina. With the increase of nitrate applied to fl ue-cured tobacco leaves, nitrate nitrogen and TSNA content after high temperature storage increased signifi cantly. These results indicated that nitrate nitrogen content was closely related to the formation of TSNAs during high temperature storage. Therefore, reduction of leaf nitrate content through agronomic measures is effective in reducing TSNA formation during storage.更多还原