【作者】 李玲燕； 徐宜民； 刘百战； 王程栋； 毕艳玖； 刘晓冰； 杨凡； 王树声；

【Author】 LI Lingyan;XU Yimin;LIU Baizhan;WANG Chengdong;BI Yanjiu;LIU Xiaobing;YANG Fan;WANG Shusheng;Tobacco Research Institute of CAAS, Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture;Graduate School of Chinese Academy of Agricultural Sciences;College of Agriculture and Plant Protection, Qingdao Agricultural University;Shanghai Tobacco Group Co., Ltd.;

【机构】 中国农业科学院烟草研究所农业部烟草生物学与加工重点实验室； 中国农业科学院研究生院； 青岛农业大学农学与植物保护学院； 上海烟草集团有限责任公司；

【摘要】 为明确不同生态区域烤烟关键香气物质种类,并依据香气物质对不同生态区域进行分类,采用正己烷常温萃取法和液相-气相-质谱联用技术对不同烤烟风格6个生态区域烤烟烟叶香气物质进行测定。结果表明,不同生态区域烟叶有14种香气物质差异显著,贵州中部山区烟叶中巨豆三烯酮等8种香气物质含量显著高于其他产区。主成分分析表明,巨豆三烯酮、茄酮和降茄二酮是不同风格烤烟的关键香气物质;以贵州中部山区烟叶香气物质的主成分得分排名最高,云贵高原产区排名最低。通过主成分聚类分析将6个生态区划分为3类,第1类以贵州中部山区为主;第2类以秦巴山区为主;第3类以武陵山区为主,而山东产区、东北产区和云贵高原产区的取样点分别在第2、3类中分布。基于烟叶香气物质含量的不同生态区烤烟评价和分类是可行的。更多还原

【Abstract】 The main purpose of this study was to explore the key aroma in flue-cured tobacco leaves, and to classify different ecological regions tobacco based on this. Seventeen aroma were analyzed by N-hexane solvent extraction and liquid chromatography-gas chromatography-mass spectrometry(LC-GC-MS) method with 115 flue-cured tobacco samples collected from 25 sampling points in six ecological regions. Principal component analysis and cluster analysis were conducted on the determination results of aroma. Results showed that the contents of fourteen aroma components were significantly different among flue-cured tobacco leaves from different ecological regions. Moreover, eight aroma components including megastigmatrienonel in flue-cured tobacco leaves of mountainous areas of central Guizhou were significantly higher than the other regions. Principal component analysis suggested that the key of aroma components in flue-cured tobacco leaves included megastigmatrienonel, solanone and norsolandione. The highest score of principal component was from the mountainous areas of central Guizhou, while the lowest score was from Yunnan, Guizhou plateau production areas. Using principal component-cluster analysis method based on the contents of aroma components in flue-cured tobacco leaves, the six ecological regions tobacco in this study could be divided into three clusters, with the first one represented by the mountainous areas of central Guizhou, the second one represented by the mountainous areas of Qinba, and the third one represented by the mountainous areas of Wuling. In addition, the sampling points of Shandong production areas, Northeast production areas and Yunnan, Guizhou plateau production areas belonged to the second and third cluster respectively. Aroma components can be used in evaluating and classification of flue-cured tobacco from different ecological regions.更多还原