【作者】 刘超英； 汪霞； 高洪； 严玉霖； 富国文； 赵汝；

【Author】 Liu Chao-ying;Wang Xia;Gao Hong;Yan Yu-lin;Fu Guo-wen;Faculty of Animal Science and Technology,Yunnan Agricultural University;

【机构】 云南农业大学动物科学技术学院；

【摘要】 临床上常用氟喹诺酮类药物治疗大肠杆菌引起的感染性疾病,近年来随着药物使用频率和剂量的增加,大肠杆菌对氟喹诺酮类药物的耐药性也逐渐增强。为更好的指导临床用药,本实验利用三重PCR反应体系对aac（6’）-Ib-cr、qnrD和qepA三个耐药基因进行了检测,成功建立了一种快速、准确的细菌耐药性检测方法。结果显示,本实验建立的多重PCR技术与单重PCR实验符合率达到90%以上,特异性高,重复性好,敏感性最大可以达到10^-4倍浓度稀释,不仅为大量临床样品的检测节约了时间和经济成本,也为检测大肠杆菌对氟喹诺酮类药物的耐药基因提供了新的技术手段。更多还原

【Abstract】 FQs is commonly used in the treatment of infectious diseases which are caused by Escherichia coli.In recent years,with the increasement of the frequency and dosage of drugs,the resistance of Escherichia coli to FQs has gradually increased.To better guide clinical medication,this experiment had used triple PCR reaction system and detected aac（6’）-Ib-cr,qnrD and qepA resistance genes,and successfully established a rapid and accurate detection method of bacterial resistance.The results showed that the established multiplex PCR technique and single PCR experimental coincidence rate reached more than 90%.Moreover,multiplex PCR’s specificity is high,and repeatability is good. It’s sensitivity can reach 10^-4 times of dilution,which not only saves time and cost for a large number of clinical samples,but also provide a new technique for detection of Escherichia coli fluoroquinolone resistance gene.更多还原