【作者】 雷涛； 杨磊； 张菊梅； 谢腾飞； 吴清平；

【Author】 Lei Tao;Yang Lei;Zhang Jumei;Xie Tengfei;Wu Qingping;State Key Laboratory of Applied Microbiology Southern China,Guangdong Institute of Microbiology;

【机构】 广东省微生物研究所省部共建华南应用微生物国家重点实验室；

【摘要】 本研究拟对副溶血性孤菌生物被膜形成的影响因素进行探究,建立副溶血性弧菌生物被膜体外模型。以副溶血性弧菌模式菌株ATCC17802为研究对象,采用改良结晶紫染色法测定其在不同培养温度(4-37℃)、pH(4-10)、NaCl浓度(0-8%)及接菌量条件下的生物被膜形成能力。结果显示,在25℃、30℃和37℃条件下,ATCC17802具有较强的成膜能力,在25℃时,成膜能力最强。在pH6-8范围内,ATCC17802的成膜能力较强,在pH6时,成膜能力最强。在NaCl浓度为0-4%时,ATCC17802的成膜能力较强,在1%时,成膜能力最强。细菌接种量对ATCC17802的成膜能力没有显著的影响。本研究探明了不同因素对副溶血性弧菌成膜能力的影响,明确了构建副溶血性弧菌生物被膜体外模型的最佳务件,为研究副溶血性弧菌生物被膜引起的食品安全问题奠定了基础。更多还原

【Abstract】 The objective of the present study is to investigate the biofilm-forming influence factors of Vibrio parahamealyticus,and establish the biofilm model of Vibrio parahamealyticus in vitro.The reference strain ATCC17802 was chosen for biofilmforming analysis using modified crystal violet staining method under various factors,such as different culture temperature(4-37℃),pH(4-10),NaCl concentration(0-8%),and inocution amount.As the result showed,at 25℃,pH6,and1%NaCl,the biofilm-forming ability of ATCC17802 is the best.However,different dilution of the bacterial inoculum on biofilm-formation is not obvious.Our research revealed the different influence factors on biofilm-formation of Vibrio parahamealyticus,established the biofilm model of Vibrio parahamealyticus in vitro,which have a certain reference value on the study of the relation between biofilm of Vibrio parahamealyticus and food safety.更多还原