【作者】 章检明； 易华西； 张兰威；

【Author】 Zhang Jianming;Yi Huaxi;Zhang Lanwei;Department of Food Science and Engineering,Harbin Institute of Technology;

【机构】 哈尔滨工业大学化工学院食品科学与工程系；

【摘要】 乳酸菌细菌素是一类由核糖体合成,具有抑菌作用的多肽或蛋白类物质。目前除nisin外,其它乳酸菌细菌素由于产量偏低不能实现工业化生产,提高乳酸菌细菌素合成量对解决商业化生产的技术瓶颈具有重要的意义。课题组前期已从我国传统发酵食品中分离出一株具有能合成广谱、性质稳定细菌素Lac-B23的植物乳杆菌B23,但产量较低。本研究利用微生物共培养技术来提高植物乳杆菌B23合成细菌素Lac-B23的产量。结果表明,单增李斯特氏菌和金黄色葡萄球菌与植物乳杆菌B23共培养后能够增加Lac-B23合成量。并且诱导菌初始接菌量影响着细菌素Lac-B23合成。当单增李斯特菌的初始接菌量低于10~3 CFU/mL或金黄色葡萄球菌的初始接菌量在10~4-10~5 CFU/mL之间时才表现出诱导作用,高于这个闽值反而会抑制细菌素Lac-B23的合成。诱导分子没有释放到诱导菌发酵液中,诱导菌灭活菌体细胞对细菌素Lac-B23的合成量没有诱导作用。植物乳杆菌B23只有与诱导菌活体细胞共培养时才能增加细菌素Lac-B23的合成。更多还原

【Abstract】 Bacteriocins from lactic acid bacteria are ribosomally synthesized,antimicrobial peptides.So far,however,only nisin carry out the commercial production.Most of bacteriocins are still in research stage because of low production levels.Study of improving bacteriocin production is important for industrial production.In our laboratory,we had got a strain called Lactobacillus plantarum B23 from Chinese traditional fermented milk,which could steadily produce bacteriocin Lac-B23,but production level was low.Therefore,we made use of co-culture technology to increase bacteriocin Lac-B23 production.The results showed that bacteriocin Lac-B23 production could be increased when Lactobacillus plantarum B23 was co-cultivated with Listeria monocytogenes and Staphylococcus aureus,and initial induction bacteria concentration strongly affected bacteriocin production.In addition,we proved that inducers were no released into medium,and only live induction bacteria could improve bacteriocin production.更多还原