【作者】 陈超翔； 陈晨； 周颖星； 朱少彬； 张文强； 颜晓梅；

【Author】 Chaoxiang Chen;Chen Chen;Yingxing Zhou;Shaobin Zhu;Wenqiang Zhang;Xiaomei Yan;The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University;

【机构】 厦门大学化学化工学院化学生物学系谱学分析与仪器教育部重点实验室化学生物学福建省重点实验室；

【摘要】 纳米靶向给药系统不仅可以增加药物在病变组织的浓度和疗效,而且能最大限度地降低药物的毒副作用,是当今纳米药物研发的重点发展方向。靶向纳米药物表面配体的数量、密度及其分布是研究纳米颗粒与细胞相互作用的重要参数,也是衡量靶向纳米药物质量的一个重要指标,因此需要发展单颗粒水平的配体密度定量表征方法。利用实验室研制的超高灵敏流式检测装置(HSFCM),我们发展了一种脂质体表面配体定量表征的快速方法。以叶酸脂质体为模型,我们采用荧光标记的叶酸受体对样本进行染色,使用粒径和荧光当量已知的二氧化硅纳米颗粒作为标准品,通过对单个叶酸脂质体的散射光和荧光信号的同时检测,建立了靶向纳米药物表面配体密度及其分布的单颗粒水平表征方法。该方法将为靶向纳米药物的合成优化、质量控制及细胞摄入研究等提供快速、高效的定量表征技术。更多还原

【Abstract】 Targeted nanomedicines can not only increase the therapeutic effect in lesion tissues but also significantly decrease the side effect. The density of surface ligand along with its distribution is a major factor affecting the interaction between nanoparticles and cells, and also an important parameter in the quality assessment of targeted nanomedicines. Therefore, an efficient characterization method that can analyze this feature at the single-particle level is in high demand. Taking folate-liposomes as the model system, we report the development of a rapid and quantitative method for the density characterization of surface ligand using a laboratory-built high-sensitivity flow cytometer(HSFCM). This novel method aims to meet the characterization challenge of targeted nanomedicine and promote its development.更多还原