【作者】 彭晓； 叶帅； 熊茂真； 袁淑怡； 严伟； 祝铭； 宋军； 屈军乐；

【Author】 Xiao Peng;Shuai Ye;Maozhen Xiong;Shuyi Yuan;Wei Yan;Ming Zhu;Jun Song;Junle Qu;Key Lab of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering, Shenzhen University;

【机构】 深圳大学光电工程学院,光电子器件与系统(教育部/广东省)重点实验室；

【摘要】 近年来,上转换纳米颗粒(UCNPs)由于其优越的光学性能已经在生物医学领域起到越来越重要的作用,因此需要深入了解UCNPs的细胞毒性和生物兼容性,特别是其对免疫细胞活性的影响[1-3]。我们首先利用热分解法合成了一系列的Yb/Ho掺杂的Na YF4纳米颗粒,然后以RAW264.7细胞为体外免疫活性研究模型,研究不同类型的UCNPs对细胞活性、细胞凋亡及炎症反应的影响及剂量-效应关系,在此基础上进一步研究UCNPs诱发细胞毒性效应和影响免疫活性可能的分子机制,为其进一步生物学应用提供了有效信息。更多还原

【Abstract】 Recently, the excellent optical properties of upconversion nanoparticles（UCNPs） have attracted more and more research interest in biological and biomedical applications, which demands a more comprehensive understanding of their cytotoxicity and biocompatibility, especially their immunomoulatory effects ^[1-3]. Here, a series of Na YF4 UCNPs were synthesized by using a facile thermal decomposited method. In the following studies, the UCNPs were applied onto an in vitro immune cell model, the mouse macrophage Raw264.7 cells, to carry out a series of biological tests, including cell viability assay, apoptosis test, immunomoulatory effect assays and the corresponding dose dependent effects. Finally, the underlying molecular mechanisms of the UCNPS induced cytotoxicity and modulatory effects on Raw264.7 cells were analyzed, providing useful information for further biological applications.更多还原