【作者】 胡洋； 陈艳玲； 黄观华； 杨卓鸿； 周武艺； 王朝阳；

【Author】 Yang Hu;Yanling Chen;Guanhua Huang;Zhuohong Yang;Wuyi Zhou;Chaoyang Wang;College of Materials and Energy,South China Agricultural University;College of Materials Science and Engineering,South China University of Technology;

【机构】 华南农业大学材料与能源学院； 华南理工大学材料科学与工程学院；

【摘要】 含微球的多孔支架由于其独特的结构与性能,可同时装载多种药物,使其在药物运载领域有着重要的应用价值。本文采用羟基磷灰石粒子（HAp）稳定的水包油型Pickering乳液（水相含海藻酸钠,油相含聚乳酸）作为模板,通过水相海藻酸钠与Ca²⁺的凝胶化作用固定乳液模板,经冷冻干燥便可"一锅法"制得载聚乳酸微球的海藻酸钙/HAp多孔支架。采用扫描电子显微镜对多孔支架的形貌进行了表征,并对其细胞相容性、体外药物控释性能进行了研究。研究结果表明微球分散在多孔支架的孔洞内或镶嵌在孔壁上。多孔支架的孔结构及微球的大小可以通过改变乳液制备条件来调控。多孔支架能促进细胞增殖,具有良好的生物相容性。将多孔支架用作双药物载体,布洛芬装载到微球中,牛血清蛋白装载到支架基质中。药物释放研究结果表明多孔支架对装载的两种药物都具缓释作用,其中对疏水性的布洛芬缓释效果更好。更多还原

【Abstract】 The microsphere-incorporated porous scaffolds have received much interest in drug delivery application,because of their special structures and performances.Herein,poly（L-lactic acid） microsphere-incorporated calcium alginate/hydroxyapatite（HAp） porous scaffolds are prepared via "one-pot method" basing on HAp-stabilized O/W Pickering emulsion templating,which possesses alginate in aqueous phase and poly（L-lactic acid） in oil phase.The emulsion templating is firstly solidified by gelation of alginate with Ca²⁺,and then freeze-dried to obtain microsphere-incorporated porous scaffolds.The results indicated that the structures of scaffolds and sizes of microspheres can be altered by the changing fabrication conditions.BMSCs cells can well proliferate on the scaffolds,showing the good biocompatibility of the scaffolds.Additionally,model drug ibuprofen（IBU） and bovine serum albumin（BSA） are loaded into the microspheres and scaffold matrix,respectively.The results of in vitro release studies show that IBU has a sustained release while BSA has a rapid release in the drug-loaded scaffolds.更多还原