【作者】 张礼飞； 郭霞；

【Author】 Lifei zhang;Xia Guo;School of Chemistry and Chemical Engineering, Yangzhou University;

【机构】 扬州大学化学化工学院；

【摘要】 目前,研究人员多研究纯品酶的性质,而对于植物等复杂机体中酶性质的调控研究尚不多见。本文研究了表面活性剂十二烷基三甲基溴化铵(DTAB)和十六烷基三甲基溴化铵(CTAB)对苹果中多酚氧化酶(PP0)活性的调控作用,并结合pH、离子强度、温度等因素对该体系中PP0的活性进行了研究,探讨了表面活性剂对复杂机体中酶活性的调控效能。我们发现使用CTAB时,当CTAB浓度为0.3 mM、底物缓冲溶液pH为7时,PPO酶活性可提高75%左右。使用DTAB时,当DTAB浓度为15 mM、底物缓冲溶液pH为7.5时,粗酶活性可提高40%。更多还原

【Abstract】 Currently,researchers usually focus on studying the properties of purified enzyme,and how to modulate the properties of enzyme in organism directly is limited.In this paper,we will modulate polyphenol oxidase(PPO)activity in apple juice by using surfactants dodecyltrimethylammonium bromide(DTAB) and cetyltrimethylammonium bromide(CTAB).By combining the effects of pH,ionic strength and temperature on the enzyme activity,we can discuss the regulation efficiency of surfactant on PPO activity in apple juice.It has been found that when CTAB concentration is 0.3 mM and pH is 7,PPO activity is increased by 75%,and when DTAB concentration is 15 mM and pH is 7.5,PPO activity is increased by 40%.更多还原