【作者】 冯骥； 王文景； 海欣； 于永亮； 王建华；

【Author】 Ji Feng;Wen-Jing Wang;Xin Hai;Yong-Liang Yu;Jian-Hua Wang;Research Center for Analytical Sciences, Northeastern University;

【机构】 东北大学分析科学研究中心；

【摘要】 目前,采用安全易得的原料,通过简单的合成步骤和少量的化学试剂消耗,制备高性能、低毒性的荧光探针的方法,受到广泛关注¹。本研究以蚕蛹为原料,无需加入化学试剂,通过微波消解,一步法制备了高量子产率、低毒性、具有良好水溶性与光稳定性的荧光碳点。通过TEM、XRD、FT-IR、XPS、紫外和荧光对碳点的形貌和光学特性进行了表征,其平均粒径为19 nm,碳、氧、氮的含量分别为71.32%、22.96%和5.72%,以硫酸奎宁为参比的相对量子产率为46%,激发/发射波长位于350/420 nm,且荧光发射表现出激发依赖性。进一步研究表明,在碳点浓度高达15 mg mL^-1时,依然呈现出很低的细胞毒性。倒置荧光显微镜观察发现,在不同波长激发下,该碳点可呈现出明亮的蓝色、绿色和红色,展现了其在细胞成像应用中的良好前景²。更多还原

【Abstract】 Carbon dots with a high quantum yield have been synthesized by a green one-pot approach under microwaves with silkworm chrysalis, without using any other chemicals/reagents. The morphology and optical properties of the resultant CDs are characterized by TEM, XRD, FT-IR, XPS, UV-vis and photoluminescence. The excitation/emission wavelength is 350/420 nm, and a quantum yield of 46%. In addition to excellent solubility and stability in aqueous medium, the SC-CDs exhibit a large Stokes shift of 70 nm. It is further demonstrated that the SC-CDs exhibit a low cytotoxicity at a higher concentration of 15 mg mL^-1 and they can display bright blue, green and red colors under an inverted fluorescence microscope, showing their vast potential in bioimaging.更多还原