【作者】 赵宗保；

【Author】 Zongbao K.Zhao;Dalian Institute of Chemical Physics,CAS;

【机构】 中国科学院大连化学物理研究所；

【摘要】 脂质代谢与细胞的生物学功能密切相关。研究酵母脂质代谢不仅对深入认识脂肪酸和异戊烯代谢途径具有重要意义,而且可用于建立干预脂质代谢的新方法及构建先进的"细胞工厂"。某些微生物具有过量积累脂质超过细胞干重20wt%的特殊潜力。担子菌属的圆红冬孢酵母是脂质代谢研究的重要模式材料,其积累的脂质主要包括长链脂肪酸甘油脂和萜类化合物。我们采用多组学方法,建立了圆红冬孢酵母高质量基因组数据库,研究不同营养条件下红酵母的生长代谢行为,发现氮/磷缺乏诱导油脂积累涉及全局性响应,包括表达上调氮/磷代谢和自噬相关基因,下调核酸和蛋白质合成相关基因,抑制细胞增殖。完成了脂滴蛋白质组学分析,鉴定出一种新颖的脂滴蛋白,发现其转录和蛋白水平变化与油脂积累紧密关联,在模式宿主中表达该脂滴蛋白可促进脂质积累。测试了系列小分子化合物对产油酵母生长代谢的影响,发现多种干预脂肪酸/异戊烯代谢的化合物。本报告将介绍上述研究成果。更多还原

【Abstract】 While lipid metabolism is essential to life,only a small portion of microorganisms can accumulate lipids to over 20% of their dry cell mass.The basidiomycetous yeast Rhodosporidium toruloides is an exceptional lipids producer which can make storage lipids mainly in the form of triacylglycerols and terpenoids.We took a trans-omic approach to study R.toruloides and established its functional genomic database.We found that lipid accumulation under nutrient limitation is regulated at different levels,including transcriptional,translational and beyond.We performed proteomic analysis of lipid droplets(LD),identified a number of LD-associated proteins,and demonstrated that these proteins were positively correlated with lipid accumulation.We cultivated R.toruloides in the presence of various small compounds and found a number of active candidates.This presentation will summarize our recent progress in this area.更多还原