【作者】 孙海慧； 吴昊； 尹振浩； 尹成日；

【Author】 Haihui Sun;Hao Wu;Zhenhao Yin;Chengri Yin;Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules,Ministry of Education,Yanbian University;Analytical and Testing Center,Yanbian University;

【机构】 延边大学长白山生物资源教育部重点实验室； 延边大学分析测试中心；

【摘要】 丙烯酸甲酯是一种重要的化工原料和溶剂广泛应用于各行各业[1]。同时,作为一种挥发性有机化合物,严重影响生态环境和人类健康[2]。本研究采用PCR-DGGE法,对一个用于丙烯酸甲酯废气处理的三段式生物滴滤池中的生物膜的16S r DNA V3区扩增,扩增产物经过变形梯度凝胶电泳,回收测序鉴定,并与活性污泥做了对比。结果表明,丙烯酸甲酯废气浓度对细菌群落影响较大。生物滴滤池最下层的优势细菌有7中,他们是V.paradoxus,D.koreensis,P.suwonensis,A.caulinodans,H.denitrificans,Hyphomicrobium sp.和C.testosterone.更多还原

【Abstract】 Methyl acrylate is a widely used raw chemical material and it is toxic in humans.In this study,the bacterial community of a methyl acrylate removal biotrickling filter was analyzed by PCR-DGGE.The V3 variable region of the biofilm’s 16 S r DNA was amplified and eluted,re-amplified,and sequenced.The result show that the concentration of methyl acrylate had a significant influence on changing of bacterial community.Seven species of species contributed to the degradation methyl acrylate.Those species were V.paradoxus,D.koreensis,P.suwonensis,A.caulinodans,H.denitrificans,Hyphomicrobium sp.and C.testosterone,respectively.更多还原