Study on the molecular mechanism of trichloroethylene-induced allergic dermatitis

【作者】 徐新云； 刘月峰； 李学余； Xiaoyun Qin； Kanlang Mao；

【Author】 Xinyun Xu;Yuefeng Liu;Xueyu Li;Xiaoyun Qin;Kanlang Mao;Department of toxicology,Shenzhen center for disease control and prevention;

【机构】 深圳市疾病预防控制中心毒理学重点实验室；

【摘要】 Trichloroethylene(TCE) is one of the common organic solvents that has been widely used in cleaning or degreasing of metal products and electronic products.However,hundred cases of hypersensitivity dermatitis have occurred after the workers were occupationally exposed to TCE in China for the past decad.The purpose of this study was to investigate mRNA expression of hepatic metabolic enzyme genes,immune-related genes,apoptosis genes and oncogenes in patients with allergic dermatitis induced by trichloroethylene.12 typical patients with TCE-induced hypersensitivity dermatitis were investigated as the study cases,peripheral blood samples were taken from patients and control,real-time fluorescent PCR assay was applied for detection of mRNA expression of hepatic metabolic enzyme genes,immune-related genes,apoptosis genes and oncogenes.It was found that the relative levels of mRNA expression of CYP1A2,CYP2E1,CYP3A4,CYP2C9 increased by723%,318%,385%,216%,respectively,when compared with control(P<0.01 or P<0.05);Foxp3,GATA3 and CTLA4 mRNA expression increased by 104%,106%,253%,respectively,in TCE patients when compared with control(P<0.01);T-bet expression decreased by 44%when compared with control(P<0.01);These findings indicate that some immune-related genes and hepatic metabolic enzyme genes might play an important role in the process of trichloroethylene-induced allergic dermatitis.更多还原

【Abstract】 Trichloroethylene(TCE) is one of the common organic solvents that has been widely used in cleaning or degreasing of metal products and electronic products.However,hundred cases of hypersensitivity dermatitis have occurred after the workers were occupationally exposed to TCE in China for the past decad.The purpose of this study was to investigate mRNA expression of hepatic metabolic enzyme genes,immune-related genes,apoptosis genes and oncogenes in patients with allergic dermatitis induced by trichloroethylene.12 typical patients with TCE-induced hypersensitivity dermatitis were investigated as the study cases,peripheral blood samples were taken from patients and control,real-time fluorescent PCR assay was applied for detection of mRNA expression of hepatic metabolic enzyme genes,immune-related genes,apoptosis genes and oncogenes.It was found that the relative levels of mRNA expression of CYP1A2,CYP2E1,CYP3A4,CYP2C9 increased by723%,318%,385%,216%,respectively,when compared with control(P<0.01 or P<0.05);Foxp3,GATA3 and CTLA4 mRNA expression increased by 104%,106%,253%,respectively,in TCE patients when compared with control(P<0.01);T-bet expression decreased by 44%when compared with control(P<0.01);These findings indicate that some immune-related genes and hepatic metabolic enzyme genes might play an important role in the process of trichloroethylene-induced allergic dermatitis.更多还原