【作者】 郭志廷； 王玲； 李春； 梁剑平； 尚若锋； 杨珍；

【Author】 GUO Zhi-ting;WANG Ling;LI Chun;LIANG Jian-ping;SHANG Ruo-feng;YANG Zhen;Key Laboratory of Veterinary Pharmaceutics Discovery,Ministry of Agriculture,Key Laboratory of New Animal Drug Project,Gansu Province,Lanzhou Institute of husbandry and Pharmaceutical sciences of CAAS;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences;

【机构】 中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室； 中国中医科学院中药研究所；

【摘要】 目的:确定常山总碱超声提取的最佳工艺条件。方法:采用稀盐酸溶媒、超声提取法从中药常山中分离常山总碱,应用正交试验法确定其最佳提取工艺条件,采用分光光度法测定常山碱含量。结果:常山总碱最佳提取工艺为:提取温度50℃,料液比1:5,提取时间1 h;上述条件下常山总碱的提取率为2.57%,其中常山碱的含量为0.30%。结论:超声提取法用于常山总碱的分离,具有省时、节能、提取率高等优点。更多还原

【Abstract】 Objective To optimize ultrasonic extraction technology of total dichroa alkaloid(TDA).Methods:TDA of Dichroa febrifuga Lour were separated by dilute hydrochloric acid and ultrasonic extraction method.Its extraction process were optimized by orthogonal design.Dichroine content were determined by spectrophotometry.Results:The optimization process of TDA as follows:temperature 50℃,solid-liquid ratio 1:5,time 1 h.Under above conditions,TDA yield were up to2.57%) and the dichroine content were up to 0.30%.Conclusion:Isolation process of TDA is feasible by ultrasonic extraction method,which have advantages of time-saving,energy conservation and higher extraction rate.更多还原