类风湿关节炎患者外周血单核细胞高表达Siglec-1及其在单个核细胞对自身抗原反应中的作用（英文）

【作者】 熊怡淞； 程悦； 林秋水； 吴艾霖； 俞娟； 李畅； 孙懿； 仲人前； 吴丽娟；

【机构】 成都军区总医院临床实验医学研究与保障中心； 成都军区总医院肾内科； 中国人民解放军411医院骨科； 第二军医大学附属长征医院实验诊断科； 第二军医大学附属长海医院实验诊断科；

【摘要】 Objectives Elevated expression of Siglec-1 on circulating monocytes has been reported in some inflammatory and autoimmune diseases,but its expression and role in rheumatoid arthritis(RA)has not been elucidated.Methods Siglec-1protein and mRNA levels in 42 RA patients,39 osteoarthritis patients,28 SLE patients and 42 normal controls were determined by flow cytometry and QRT-PCR,respectively.In addition,ten patients with active RA were received DMARDs for 12 weeks and the frequencies of Siglec-1(+)cells and DAS28 were assessed before and after therapy.Furthermore,TNF-α,IFN-γand typeⅡcollagen were used to up-regulate Siglec-1 and PBMCs from different groups were stimulated with mitogens or antigens and cells proliferation and cytokines production were determined.Results The protein and mRNA levels of Siglec-1 on PBMCs and monocytes in RA patients were significantly higher than those in OA patients and healthy controls.Moreover,the expression of Siglec-1 protein on PBMCs was positively correlated with DAS28,ESR,hs-CRP and IgM-RF,but not with anti-CCP antibody.Interestingly,Siglec-1 expression was decreased in parallel with the decrease of DAS28 after12-week antirheumatic treatment.Furthermore,TNF-α,IFN-γand typeⅡcollagen can up-regulate Siglec-1 on PBMCs.Elevated PBMCs proliferation and pro-inflammatory cytokines production to collagen stimulation in RA patients reduced when Siglec-1 was inhibited by anti-Siglec-1 antibody.Conclusions Elevated Siglec-1 expression on PBMCs and monocytes can potentially serve as a biomarker for monitoring disease activity in RA.Siglec-1 may also play a proinflammatory role in stimulating lymphoeytes proliferation and activation in RA.更多还原

【Abstract】 Objectives Elevated expression of Siglec-1 on circulating monocytes has been reported in some inflammatory and autoimmune diseases,but its expression and role in rheumatoid arthritis(RA) has not been elucidated.Methods Siglec-1protein and mRNA levels in 42 RA patients,39 osteoarthritis patients,28 SLE patients and 42 normal controls were determined by flow cytometry and QRT-PCR,respectively.In addition,ten patients with active RA were received DMARDs for 12 weeks and the frequencies of Siglec-1(+) cells and DAS28 were assessed before and after therapy.Furthermore,TNF-a,IFN-y and type II collagen were used to up-regulate Siglec-1 and PBMCs from different groups were stimulated with mitogens or antigens and cells proliferation and cytokines production were determined.Results The protein and mRNA levels of Siglec-1 on PBMCs and monocytes in RA patients were significantly higher than those in OA patients and healthy controls.Moreover,the expression of Siglec-1 protein on PBMCs was positively correlated with DAS28,ESR,hs-CRP and IgM-RF,but not with anti-CCP antibody.Interestingly,Siglec-1 expression was decreased in parallel with the decrease of DAS28 after12-week antirheumatic treatment.Furthermore,TNF-α,IFN-γ and type Ⅱ collagen can up-regulate Siglec-1 on PBMCs.Elevated PBMCs proliferation and pro-inflammatory cytokines production to collagen stimulation in RA patients reduced when Siglec-1 was inhibited by anti-Siglec-1 antibody.Conclusions Elevated Siglec-1 expression on PBMCs and monocytes can potentially serve as a biomarker for monitoring disease activity in RA.Siglec-1 may also play a proinflammatory role in stimulating lymphocytes proliferation and activation in RA.更多还原