The clinical significance of serum cytokine profile in cancers

【作者】 田亚平；

【机构】 中国人民解放军总医院；

【摘要】 The previous studied confirmed that inflammation were involved in the development of cancer.In this study serum cytokines and tumor markers have been studies and explore their clinical utility of biomarker profile.Serum cytokines of GM-CSF,IFN γ,IL-10,IL-1β,IL-2,IL-4,IL-6,IL-8,MCP-1 and TNFa have been analyzed by Luminex technology(Luminex200).Serum tumor marker of CEA,AFP,CA125,CA153,CA199,CA724 and CYFRA21-1 have been measured by the Electrochemoluminescence technology(Roche E170 molecular immunoassay analyzer)and SCC were detected by the Chemiluminnesent Microparticle ImmunoAssay technology(Architect i2000 SR,ABBOTT).Patients who suffer colorectal cancer,pancreatic cancer,cervical cancer,hepatocellular cancer have been involved in these studies.The results showed that serum levels of interleukin-8 and monocyte chemoattractant proteinl(MCP-1) were both significantly higher in cervical diseases patients(CIN and cervical cancer patients) than in healthy control(P<0.05).Serum IL-6 and IL-10 concentrations were significantly elevated CIN and cervical cancer patients,and cervical cancer group have more increased level compare with CIN(P<0.05).IL—1β and IL-2 is obviously increased in pancreatic cancer,and compared to benign diseases,MCP-1 decreased and GM-CSF increased.The ratio of MCP-1/GM-CSF between pancreatic benign diseases and cancer will be more helpful for the differential diagnosis.The comparative studies of benign colorectal polyps(CRP) and colorectal cancer(CRC) patients showed that serum GM-CSF,IFN-γ,IL-1β and IL-6 which were used for differentiating the CRP and early stage CRC and IL-6 had the best diagnostic value.IL-10 and IL-1β showed significant difference between early stage CRC and advance stage CRC,also in the lymph nodes metastasis present and absent.Serum IL-6,MCP-1 and TNF-α also have boon found have significant difference between different stage of CRC.The above results indicated that the level of serum cytokines is closed related with the development of variety of cancer and combined use it with tumor marker will be significantly improve the diagnostic value.更多还原

【Abstract】 The previous studied confirmed that inflammation were involved in the development of cancer.In this study serum cytokines and tumor markers have been studies and explore their clinical utility of biomarker profile.Serum cytokines of GM-CSF,IFN γ,IL-10,IL-1β,IL-2,IL-4,IL-6,IL-8,MCP-1 and TNFa have been analyzed by Luminex technology(Luminex200).Serum tumor marker of CEA,AFP,CA125,CA153,CA199,CA724 and CYFRA21-1 have been measured by the Electrochemoluminescence technology(Roche E170 molecular immunoassay analyzer)and SCC were detected by the Chemiluminnesent Microparticle ImmunoAssay technology(Architect i2000 SR,ABBOTT).Patients who suffer colorectal cancer,pancreatic cancer,cervical cancer,hepatocellular cancer have been involved in these studies.The results showed that serum levels of interleukin-8 and monocyte chemoattractant proteinl(MCP-1) were both significantly higher in cervical diseases patients(CIN and cervical cancer patients) than in healthy control(P<0.05).Serum IL-6 and IL-10 concentrations were significantly elevated CIN and cervical cancer patients,and cervical cancer group have more increased level compare with CIN(P<0.05).IL—1β and IL-2 is obviously increased in pancreatic cancer,and compared to benign diseases,MCP-1 decreased and GM-CSF increased.The ratio of MCP-1/GM-CSF between pancreatic benign diseases and cancer will be more helpful for the differential diagnosis.The comparative studies of benign colorectal polyps(CRP) and colorectal cancer(CRC) patients showed that serum GM-CSF,IFN-γ,IL-1β and IL-6 which were used for differentiating the CRP and early stage CRC and IL-6 had the best diagnostic value.IL-10 and IL-1β showed significant difference between early stage CRC and advance stage CRC,also in the lymph nodes metastasis present and absent.Serum IL-6,MCP-1 and TNF-α also have boon found have significant difference between different stage of CRC.The above results indicated that the level of serum cytokines is closed related with the development of variety of cancer and combined use it with tumor marker will be significantly improve the diagnostic value.更多还原