【作者】 王茂臣； 蔡晨亮； 屠娟； 郭霞生； 章东；

【Author】 WANG Mao-chen;CAI Chen-liang;TU Juan;GUO Xia-sheng;ZHANG Dong;Key Laboratory of Modern Acoustics, Institute of Acoustics, Nanjing University;

【机构】 南京大学声学研究所近代声学教育部重点实验室；

【摘要】 声孔效应作为实现基因/药物传输的有效手段正被广泛研究。但是涉及到声孔效应过程中的物理机制的研究仍然不足。由靠近弹性壁的振荡包膜微气泡产生的微流被认为是声孔效应过程中最重要的物理机制之一。本文对靠近弹性壁的振荡包膜微气泡产生的微流剪切力进行了模拟,同时考虑到了微泡包膜的边界效应和非线性的流变力学性质。结果表明,减小弹性壁杨氏模量,气泡和弹性壁更近时,或声压增大,会出现更大的剪切力。因此为了获得更有效的声孔效应结果,具有更软或者更少粘性的包膜的微气泡将是更好的选择,并且把微泡尽可能靠近细胞。更多还原

【Abstract】 Sonoporation mediated by microbubbles is being extensively studied as a promising technology to facilitate gene/drug delivery to cells. However, the theoretical study regarding the mechanisms involved in sonoporation is still in its infancy.Microstreaming generated by pulsating microbubble near the cell membrane is regarded as one of the most important mechanisms in the sonoporation process. Here, based on an encapsulated microbubble dynamic model with considering nonlinear rheological effects of both shell elasticity and viscosity, the microstreaming velocity field and shear stress generated by an oscillating microbubble near the cell membrane are theoretically simulated. The results show that reducing the Young’s modulus of the elastic wall, reducing the distance between the bubble and the elastic wall, or increasing the acoustic pressure, larger shear stress can be obtained. The results suggest that microbubbles with softer and less viscous shell materials might be preferred to achieve more efficient sonoporation outcomes, and it is better to have bubbles located in the immediate vicinity of the cell membrane.更多还原