【作者】 张晓娜； 李姝熳； 王翠翠； 田华； 王蔚； 汝少国；

【Author】 Xiaona Zhang;Shuman Li;Cuicui Wang;Hua Tian;Wei Wang;Shaoguo Ru;College of Marine Life Sciences,Ocean University of China;

【机构】 中国海洋大学海洋生命学院；

【摘要】 海胆早期发育由多种递质系统共同调控,有机磷农药是如何通过不同的递质系统而发挥神经毒性作用的机制目前尚不清楚。在课题组前期研究基础上,本研究检测了久效磷农药对马粪海胆胚胎/幼虫体内胆碱能系统发育关键时期乙酰胆碱酯酶、胆碱乙酰转移酶活性与分布的影响,以及对多巴胺(DA)能系统发育的不同时期DA含量及其I型受体(DRD1)表达量、DA代谢过程关键因子(TH、DAT、MAO表达量/活性)的影响。结果表明:在胚胎运动行为主要受DA调控的早期发育阶段(12-hpf囊胚早期、15-hpf浮游囊胚期),久效磷农药主要通过上调DA含量和/或干扰DA代谢而非影响血清素(5-HT)代谢(前期研究结果)而干扰海胆胚胎运动行为;在DA、5-HT、乙酰胆碱(ACh)等递质共同参与调控海胆形态发生和运动行为的原肠胚至二腕幼虫期,久效磷主要通过在神经递质系统发育关键时期,干扰DA、ACh、5-HT等递质代谢而扰乱这些递质所调控的海胆早期发育。因而,久效磷农药在神经递质系统发育的关键期,对不同递质含量或代谢的扰乱是其致海胆胚胎神经发育毒性的主要原因。更多还原

【Abstract】 Multiple neurotransmitters play important roles in regulating early development of sea urchin, but it is unclear how organophosphate pesticides exert neurotoxicity via disrupting these neurotransmitters. In this study, effects of monocrotophos(MCP) pesticide on activity and distribution of acetylcholinesterase(ACh E) and choline acetyltransferase(Ch AT) and on dopamine(DA) metabolism were evaluated in Hemicentrotus pulcherrimus embryos or larvae were examined. Results showed that MCP pesticide elevated DA levels and disrupted DA metabolism at the blastula stage when swimming activity was mainly regulated by DA. From gastrula to plutei, MCP affected early development of sea urchin mainly by disrupting DA,acetylcholine(ACh), and 5-HT metabolism at corresponding critical period during development of each neurotransmitter system. Therefore, mechanisms underlying developmental neurotoxicity of MCP pesticide to H. pulcherrimus embryos or larvae mainly involved disruption of multiple neurotransmitters at the critical stages during the development of each neurotransmitter systems.更多还原