【作者】 苏浩昌； 胡晓娟； 李卓佳； 许云娜； 徐武杰； 徐煜； 曹煜成；

【Author】 SU Haochang;HU Xiaojuan;LI Zhuojia;XU Yunna;XU Wujie;XU Yu;CAO Yucheng;Key Lab.of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture;Key Lab.of Fishery Ecology and Environment,Guangdong Province;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;

【机构】 中国水产科学研究院南海水产研究所广东省渔业生态环境重点实验室农业部南海渔业资源开发利用重点实验室；

【摘要】 抗生素耐药基因(Antibiotic resistance gene,ARG)是一种新型的环境污染物,其潜在的健康和生态风险受到广泛关注。土塘养殖是珠三角河口区凡纳滨对虾的主要养殖模式。本研究应用qPCR技术,对该养殖模式的池塘水体和水源水中常见的5种ARG的含量进行监测。结果显示,养殖池塘水中单种ARG的含量为3.98×10~1 copies/mL~8.06×10~5 copies/mL,水源水中单种ARG的含量明显高于养殖池塘水体,含量为3.89×10~2 copies/mL~2.00×10~6copies/mL。在对虾养殖初期和末期,池塘水中5种ARG的总含量分别为1.06×10~6 copies/mL和2.04×10~5 copies/mL,ARG去除率为80.8%。相应时期的水源水体中5种ARG的总含量分别为2.93×10~6 copies/mL和1.69×10~6 copies/mL,去除率为42.3%。耐药基因qnr A、qnr S与16S rRNA基因显著正相关,相关系数分别为0.57(p<0.05)和0.69(p<0.01),表明ARG含量与细菌数量显著相关。实验结果表明,经养殖过程,池塘水体中抗生素耐药基因的含量明显降低(p<0.05)。更多还原

【Abstract】 Antibiotic resistance gene(ARG) is emerging environmental pollutant. This study applied the qPCR method to monitor periodicly the amount of 5 common ARGs in the pond water and source water of Litopenaeus Vannamei farming in the Pearl River Delta. The results showed the amounts of single ARG were between 3.98×10~1-8.06×10~5 copies/mL in pond water and3.89×10~2-2.00×10~6 copies/mL in source water. The total amounts of 5 targeted ARGs were1.06×10~6 copies/mL and 2.04×10~5 copies/mL, respectively in the early stage and the late stage,and the removal rate of antibiotic-resistant gene was 80.8%. The total amounts of targeted ARGs were 2.93×10~6 copies/mL and 1.69×10~6 copies/mL, respectively in the corresponding stages of shrimp farming, and the removal rate of antibiotic-resistant gene was 42.3%. The qnr A and qnr S genes were positively correlated to the 16 S rRNA gene significantly, and the correlation coefficients were 0.57(p<0.05) and 0.69(p<0.01), suggesting that the amount of ARGs was significantly related to the amount of bacteria. The results indicated that the amount of ARGs in the pond water was significantly reduced with the process of shrimp farming(p<0.05).更多还原