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饲料中添加不同益生菌对虎龙斑生长、免疫力及抗氧化能力的影响

The Effects of Dietary Probiotics on Growth, Immune and Antioxidant Abilities of Hybrid Grouper(Epinephelus fuscoguttatus ♀ ×E. lanceolatus ♂)

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【作者】 黄灵李小梅舒琥张海发石和荣

【Author】 HUANG Ling;LI Xiao-mei;SHU Hu;ZHANG Hai-fa;SHI He-rong;School of Life Science, Guangzhou University;Guangdong Daya Bay Fisheries Development Center;

【机构】 广州大学生命科学学院广东省大亚湾水产试验中心

【摘要】 选用540尾体重为34 g左右的虎龙斑,随机分为6个组,在基础饲料中拌喂低浓度0.1%的芽孢杆菌、高浓度0.5%的芽孢杆菌、低浓度1%的光合细菌和高浓度5%的光合细菌,并以2%饲料用量的乳化鱼油包裹,再增设乳化鱼油组和对照组。60天结果表明,饲料中添加不同益生菌能够显著提高鱼的体重、末体长和增重率,降低饲料系数(P<0.05)。不同益生菌对末肝体比、末脏体比、肥满度和成活率影响较小,均无显著差异(P>0.05)。相比于对照组和乳化鱼油组,各益生菌添加组虎龙斑的血清总蛋白浓度、血清超氧化物歧化酶、血清碱性磷酸酶、血清溶菌酶活力显著升高(P<0.05);各益生菌添加组虎龙斑肝脏抗氧化酶(总抗氧化能力,过氧化氢酶、还原型谷胱甘肽)活性相比于对照组或乳化鱼油组均有显著提高,丙二醛水平显著降低(P<0.05)。结果表明,饲料中添加芽孢杆菌和光合细菌可有效促进虎龙斑的生长、提升虎龙斑的免疫力和抗氧化能力。

【Abstract】 Five hundred and forty fishes were randomly allotted to six groups. A basal diet was supplemented with bacillus at 0.1%, 0.5% feed and with photosynthetic bacteria at 1%, 5% feed,and then increase the control group and emulsified oil group. After feeding for 60 days, probiotics can significantly improve the final body weight, body length, weight gain rate and reduce the feed conversion ratio(P<0.05). Hepatosmatic index, viscerosomatic index, condition factor and survival rate have no significance(P>0.05). Compared with control group and emulsified oil group, the activities of serum total protein, superoxide dismutase, alkaline phosphatase, lysozyme of the probiotics diet groups increased significantly(P<0.05). The activities of hepatic antioxidant status(total antioxidant capacity, catalase, reduced glutathione) of the probiotics diet groups increased significantly and the malondialdehyde reduced significantly from those of the control group or emulsified oil group(P<0.05). These results indicated that dietary bacillus and photosynthetic bacteria could enhance the growth performance, significantly improve the immune,antioxidant ability.

  • 【会议录名称】 2016年中国水产学会学术年会论文摘要集
  • 【会议名称】2016年中国水产学会学术年会
  • 【会议时间】2016-11-02
  • 【会议地点】中国四川成都
  • 【分类号】S965.334
  • 【主办单位】中国水产学会、四川省水产学会
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