【作者】 丁贤； 张盼； 李来好； 殷波； 杨贤庆；

【Author】 DING Xian;ZHANG Pan;LI Laihao;YIN Bo;YANG Xianqing;Key Laboratory of Aquatic Product Processing,Ministry of Agriculture,P.R.China,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;South China Sea Institute of Oceanology,Chinese Academy of Science;

【机构】 农业部水产品加工重点实验室南海水产研究所中国水产科学研究院； 南海海洋研究所中国科学院；

【摘要】 根据细菌群体感应信号降解酶(degrading-signal enzyme,AiiA)基因设计探针引物,从海洋微生物ZD02基因组中扩增基因aiiA,筛选其阳性克隆,测序后分析其基因序列。结果表明:筛选到的阳性克隆ZD02-aii A序列全长753 bp(Genbank登录号:KC756214),存在一个开放阅读框(ORF),编码由250个氨基酸残基组成的多肽,预测分子量为28.1KDa,蛋白等电点为4.78。由该序列推导得到的氨基酸残基序列含有一个保守结构域(Lactamase-B)(34AA-235AA),并预测了AiiA的三级结构。以上研究为该序列的重组表达及其相关活性研究奠定了基础。更多还原

【Abstract】 The gene aii A encoding the signal-degrading enzyme(AiiA) in the genome of marine bacterium ZD 02 were amplified by PCR using a pair of degenerate primers designed according to the known conserved sequence of aii A. The PCR products(ZD02 aii A) were cloned,sequenced and analysed. The results showed that ZD02 aii A is 753 bp(Genbank accession number:KC756214) containing an open reading frame(ORF) which encoded a polypeptide chain AiiA of250 amino acids residues with molecular weight of 28 kDa and an isoelectric point value of 4.78.A conservative domain(Lactamase-B)(34AA-235AA) was identified in the sequence and the 3D structure of deduced AiiA protein was further predicted. This will lay the foundations for the expression of the sequence and analysis of its bio-activities.更多还原