【作者】 贾丹； 谢国驷； 黄倢； 史成银；

【Author】 JIA Dan;Xie Guosi;HUANG Jie;SHI Chengyin;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;

【机构】 农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所； 上海海洋大学水产与生命技术学院；

【摘要】 自2009年以来,急性肝胰腺坏死病(AHPND)在全球范围内爆发,其发病快,死亡率高,流行面广,给对虾养殖业造成极大的损失。本文从疑似患AHPND的中国明对虾体内分离得到一株细菌,经细菌的16S rDNA基因序列及生理生化分析,鉴定该株细菌为副溶血弧菌(Vibrio parahemolyticus),PCR检测该菌株具有pir A和pir B基因。使用该菌对中国明对虾进行了急性浸泡感染实验,感染后6h,对虾出现空肠空胃,9h肝胰腺呈浅白色,10h开始出现死亡,12h呈现爆发性死亡,18h死亡率达到100%。组织学观察发现,感染对虾的鰓、肝胰腺和肠都出现了不同程度的损伤,其中肝胰腺小管上皮细胞严重脱落,肝胰腺小管出现崩塌,呈现典型的急性肝胰腺坏死病病理症状,而对照组虾表现正常,未见病理变化。21种常用药物的药敏试验中,该菌只对头孢噻吩、头孢呋辛2种药物表现为耐药,对阿莫西林、庆大霉素、环丙沙星等19种药物敏感。本研究证实副溶血弧菌可以导致中国明对虾罹患急性肝胰腺坏死病,为进一步研究中国明对虾的急性肝胰腺坏死病提供了基础依据。更多还原

【Abstract】 Since 2009, Acute hepatopancreatic necrosis disease(AHPND) of shrimps has outbroken worldwide, lesding to tremendous losses in shrimp culture. In this study, a strain was isolated from F. chinensis with AHPND, which identified as Vibrio parahemolyticusbased on the16 S rDNA sequencing analysis and biochemical characteristics. Immersion challenge tests with F.chinensis were also employed for pathogenicity and pathology studies. empty gut, pale hepatopancreascould be observed, and a large number of deaths occurred after challenged.Subsequent histological analyses showed that there were many different changes in guts, gills,especially in hepatopancreas. The hepatopancreas tubules collapsed by massive sloughing of hepatopancreas epithelial cells, which is the typical pathological symptoms of AHPND, could be observed.更多还原