【作者】 纪锋； 徐黎明； 赵景壮； 刘淼； 卢彤岩； 尹家胜；

【Author】 JI Feng;XU Li-Ming;Zhao Jing-zhuang;Liu Miao;Lu Tong-yan;YIN Jiasheng;Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences;Shanghai Ocean University, College of Fisheries and Life Science;

【机构】 中国水产科学研究院黑龙江水产研究所； 上海海洋大学水产与生命学院；

【摘要】 传染性胰腺坏死(IPN)是给虹鳟养殖带来直接经济损失的一种高致死性疾病。课题组于2013年在中国云南省某鲑鳟鱼养殖场疑似感染IPN的濒死虹鳟仔鱼体内分离获得一株传染性胰腺坏死毒,命名为ChRtm213。本研究获得了IPNV-ChRtm213完整基因组序列,并与世界范围内其他IPNV序列进行了比较。结果表明,ChRtm213株的全基因组序列包含一个A片段(3099 bp)编码多聚蛋白VP2-VP4-VP3,和一个B片段(2789 bp)编码RNA依赖的RNA聚合酶VP1。系统发育分析表明,ChRtm213与基因1型的参考毒株聚在一起,血清型为A9(Jasper),与日本IPNV参考株AM98的同源性为96.3%(A链)和97.3%(B链)。结果表明,中国IPNV毒株可能起源于日本。ChRtm213的序列是目前为止Genbank数据库收录中国的唯一一个来源于鲑鳟鱼的IPNV全基因组序列。本研究为中国传染性胰腺坏死病毒的诊断和防控提供有效参考。更多还原

【Abstract】 Infectious pancreatic necrosis(IPN) is a significant disease of farmed salmonids resulting in direct economic losses due to high mortality in China. However, no gene sequence of any Chinese infectious pancreatic necrosis virus(IPNV) isolates was available. In the study,moribund rainbow trout fry samples were collected during an outbreak of IPN in Yunnan province of southwest China in 2013. An infectious pancreatic necrosis virus(IPNV) was isolated and tentatively named ChRtm213. We determined the full genome sequence of the IPNV- ChRtm213 and compared it with previously identified IPNV sequences worldwide. The sequences of different structural and nonstructural-protein genes were compared to those of other aquatic birnaviruses sequenced to date. The results indicated that the complete genome sequence of ChRtm213 strain contains a segment A(3099 nucleotides) coding a polyprotein VP2-VP4-VP3,and a segment B(2789 nucleotides) coding a RNA-dependent RNA polymerase VP1.更多还原