【作者】 高金伟； 习丙文； 滕涛； 陈凯； 秦婷； 谢骏；

【Author】 GAO JinWei;XI BingWen;TENG Tao;CHEN Kai;QIN Ting;XIE Jun;College of Fisheries and Life Science,Shanghai Ocean University;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences;Wuxi Fisheries College,Nanjing Agricultural University;

【机构】 上海海洋大学水产与生命学院； 农业部淡水渔业和种质资源利用重点实验室淡水渔业研究中心中国水产科学研究院； 南京农业大学无锡渔业学院；

【摘要】 为研究急性应激反应对鱼体铁含量及铁稳态相关基因的影响,以团头鲂为研究对象,通过腹腔注射皮质醇来模拟急性应激,采用分光光度法测定血清和肝脏中的铁含量,采用实时荧光定量PCR检测铁稳态相关基因的表达量。结果显示,皮质醇注射后血浆皮质醇显著升高,血浆中铁含量在4、8、10和12h显著高于对照组,而肝脏中的铁含量在4、8、10和12h时显著低于对照组。团头鲂肝脏中铁调素(hepcidin,hep)基因表达量显著增加,并分别在8h和10h有最大值,随后有所下降。肠道和肝脏中转铁蛋白(transferrin,Tf)的表达量均呈先升高后下降的趋势。结果表明,急性应激可造成鱼体铁代谢紊乱;hep和Tf在鱼体铁稳态调控和固有免疫反应中发挥着重要的作用。更多还原

【Abstract】 To study the effects of acute stress reaction on iron concentration and iron homeostasis in Megalobrama amblycephala, we stimulated stress response by intraperitoneal injection of cortisol. The iron content in serum and liver and the iron homeostasis related gene(hepcidin &transferrin) expression were detected by spectrophotometry and quantitative real-time PCR,respectively. The results showed that the content of serum cortisol immediately increased significantly after injection(P<0.05), then gradually decreased backward. The content of serum iron is higher than that in control group at 4, 8, 10 and 12 hour post injection(hpi), but the content of liver iron led to the opposite results. Hepcidin mRNA expression in liver raised remarkably,reaching the maximum at 8hpi and 10 hpi, respectively, and then descended to the normal level.The transferrin showed similar expression pattern, and reach the peak at 10 hpi. The result indicated that acute stress can be responsible for iron metabolism disorder in fish.更多还原