【作者】 刘淑集； 蔡云辉； 林娇芬； 王茵； 许旻； 潘南； 刘智禹；

【Author】 LIU Shu-ji;CAI Yun-hui;LIN Jiao-fen;WANG Yin;XU Min;PAN Nan;LIU Zhi-yu;Fisheries Research Institute of Fujian;Key Laboratory of Cultivation and High-value Utilization of Marin Organisms in Fujian Province;Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources;Engineering Technological Center of Mushroom Industry,Minnan Normal University;

【机构】 福建省水产研究所； 福建省海洋生物增养殖与高值化利用重点实验室； 福建省海洋生物资源开发利用协同创新中心； 菌物工程技术研究中心闽南师范大学；

【摘要】 为研究制备牡蛎糖原的最佳工艺条件,比较分析了碱性蛋白酶、木瓜蛋白酶、胃蛋白酶、中性蛋白酶和胰蛋白酶等5种不同蛋白酶对牡蛎进行酶解提取糖原的效果,发现以碱性蛋白酶为最优酶制剂。在对碱性蛋白酶酶解工艺进行单因素试验的基础上,应用Box-Bhenken中心组合设计和响应面分析法优化牡蛎糖原的提取条件,以pH、酶解温度和加酶量为自变量,牡蛎糖原提取率为指标,获得碱性蛋白酶水解提取牡蛎糖原的最佳工艺条件。即碱性蛋白酶的加酶量4%、pH 9、水解温度50℃,牡蛎糖原的提取率达(48.55±0.01)%。研究表明,响应面法对牡蛎糖原提取条件的优化方案合理可行,为提高牡蛎糖原的提取率提供了研究参考。更多还原

【Abstract】 To optimization the extraction conditions of oyster glycogen,5 proteases such as alkaline protease,papain,neutral protease,and trypsin were firstly compared by the hydrolyze effect of oyster glycogen.Alkaline protease was chosen to the next single factor experiment.The Box-Behnken center-united design and response surface methology(RSM) were introduced to optimize the process conditions of oyster glycogen extraction,in which,pH value,hydrolysis temperature and enzyme dosage were independent variables,oyster glycogen extraction rate was as an index.The result showed that the optimum process conditions were alkaline protease dosage4%,initial pH 9,and the hydrolysis temperature 50℃ On these conditions,the oyster glycogen extraction rate reached to(48.55±0.01)%.Conclusions as a result,the optimum project of oyster glycogen extraction by RSM was in reason and feasible,providing a theoretical basis for the elevation of oyster glycogen extraction.更多还原