【作者】 吴超； 齐振宇； 郭方其；

【Author】 WU Chao;QI Zhen-yu;GUO Fang-qi;Institute of Horticulture,Zhejiang Academy of Agricultural Sciences;Agricultural Experiment Station of Zhejiang University;

【机构】 浙江省农业科学院园艺研究所； 浙江大学农业试验站；

【摘要】 研究不同蝴蝶兰花瓣突变基因组DNA甲基化水平变化情况,探索在花瓣不同突变过程中DNA甲基化的作用。实验结果表明,淡色突变体基因组CCGG位点发生甲基化的方式主要是以双链全甲基化(CmCGG)为主,而裂纹和花边突变体因组CCGG位点发生甲基化的方式主要是以单链甲基化(CmCGG)为主。运用甲基化敏感扩增多态性(methylation se nsitive amplified polymorphism,MSAP)技术,分析DNA甲基化水平和模式改变的表观遗传变异,实验结果表明形成蝴蝶兰花边突变体的甲基化和去甲基化会高于裂纹和淡色花瓣突变体,而裂纹花瓣突变体的甲基化和去甲基化低于未甲基化,推测该类型突变体的CCGG位点甲基化不是主效引起突变的途径。更多还原

【Abstract】 Study on different petals Phalaenopsis mutation changes in DNA methylation,DNA methylation in exploring different mutations in the role of petals.The experimental results show that methylation of light colour mutant genome CCGG site is the main way to methyl double chain(CmCGG),and by the edge seam and decorative border mutants group CCGG sites methylation is the main way to single stranded methylation(CmCGG).Using methylation sensitive amplified polymorphism(methylation se nsitive amplified polymorphism MSAP) technology,the change of DNA methylation level and pattern of epigenetic variation,the experimental results show that the formation of Phalaenopsis decorative border mutants methylation and demethylation will be higher than the light colour and edge seam mutants,and the edge seam mutants ofmethylation and demethylation is lower than unmethylated,mutation pathway speculated that the mutant type of CCGG methylation is not the main effect更多还原