【作者】 隋丽； 张小玲； 孔福全；

【Author】 SUI Li;ZHANG Xiao-ling;KONG Fu-quan;Department of Nuclear Physics,China Institute of Atomic Energy;

【机构】 中国原子能科学研究院核物理研究所；

【摘要】 p53是众所周知的肿瘤抑制基因,其下游的基因PIG3是DNA损伤诱导基因,在P53蛋白的转录调控机制以及许多生理与细胞病理反应过程中发挥着重要作用,如细胞周期阻滞、凋亡和老化等,一些研究表明PIG3有望成为新的生物剂量计。本研究以不同剂量热中子和单能快中子分别辐照人类正常淋巴母细胞AHH-1,运用实时荧光定量PCR检测PIG3表达、流式细胞术检测细胞周期变化,研究了不同能量中子对AHH-1细胞的影响,为PIG3作为新的生物剂量计用于中子剂量测量研究提供实验基础。结果表明,热中子和快中子辐射均可引起PIG3表达量的快速增加,剂量响应均符合一元二次关系;快中子诱导PIG3表达在时间上先于热中子达到峰值,并且在表达量上远大于热中子。热中子和快中子均诱导AHH-1出现明显的G2/M期阻滞,且其与各自的PIG3表达在时间趋势上相一致;快中子还可诱导明显的G1期阻滞,出现的时间在G2/M期阻滞之后。快中子对AHH-1细胞的PIG3表达和细胞周期的影响均显著高于热中子辐射。更多还原

【Abstract】 p53 is a well-known tumor suppressor gene.PIG3 is downstream gene of p53 induced by DNA damage and play important role in many physiological and pathological responses,such as cell cycle arrest,apoptosis,aging and so on.Previous studies suggested that PIG3 is a promising biological dosimeter.In present study,the PIG3 expression and cell cycle redistribution of AHH-1irradiated by thermal or fast neutrons were investigated,to provide experimental basis for PIG3 as a new biological dosimeter applied in neutron dosimetry.The results showed that PIG3 expression induced by thermal or fast neutron increased in linear-quadratic correlation with dose increased,and the quantity or time of appearance for fast neutrons was much more and earlier than that of thermal neutrons.Both of them also induced G2/M arrest in similar time-response with each of PIG3 expression.Moreover,G1 arrest was also detected in fast neutron irradiated AHH-1 and followed G2/M arrest.In conclusion,the effect on AHH-1 from fast neutrons was more significant than that of thermal neutrons.更多还原