Mapping freezing tolerance quantitative trait loci in common wheat using a doubled haploid population

【作者】 周梦； Yong Zhao； Xuefang Yang； Guishun Chen； Ke Xu； Jichun Tian； 张树华； 杨学举；

【Author】 Meng Zhou;Yong Zhao;Xuefang Yang;Guishun Chen;Ke Xu;Jichun Tian;Shuhua Zhang;Xueju Yang;Huabei Key Laboratory of Crop Germplasm,Hebei Agricultural University;Shandong Agricultural University;

【机构】 Huabei Key Laboratory of Crop Germplasm,Hebei Agricultural University； Shandong Agricultural University；

【摘要】 Exposure to cold conditions is a major abiotic stress affecting crop growth and productivity.Here,quantitative trait loci(QTLs) for antioxidant enzyme activity,ratio of free water to bound water(RFB),and malondialdehyde(MDA) content in six environments were identified using a doubled haploid wheat population derived from a cross between Huapei-3 and Yumai-57 wheat cultivars.The QTLs were analyzed using an inclusive composite interval mapping approach.Consequently,35 additive QTLs for specific traits were identified,including QRFB-2 A-1,which was detected in three environments.Moreover,QPOD-2 D-2 and QPOD-1 B,which were associated with peroxidase activity,were detected in two environments,with QPOD-2 D-2 responsible for more than 10%of the phenotypic variation.Additionally,QCAT-6 D-2 on chromosome 6 D explained 16.80%of the phenotypic variation,with the positive effect contributed by Huapei-3.Furthermore,eight additive QTLs for MDA content were detected on chromosomes 1 A,2 B,3 A,4 B,5 B.2,6 B,and 7 B,including QMDA-6 B between markers Xcfa2257 and Xcfd48,which was responsible for 20.21%of the phenotypic variation.By marker-assisted selection,the major QTLs detected in this study may be valuable for improving freezing tolerancein wheat.更多还原

【Abstract】 Exposure to cold conditions is a major abiotic stress affecting crop growth and productivity.Here,quantitative trait loci(QTLs) for antioxidant enzyme activity,ratio of free water to bound water(RFB),and malondialdehyde(MDA) content in six environments were identified using a doubled haploid wheat population derived from a cross between Huapei-3 and Yumai-57 wheat cultivars.The QTLs were analyzed using an inclusive composite interval mapping approach.Consequently,35 additive QTLs for specific traits were identified,including QRFB-2 A-1,which was detected in three environments.Moreover,QPOD-2 D-2 and QPOD-1 B,which were associated with peroxidase activity,were detected in two environments,with QPOD-2 D-2 responsible for more than 10%of the phenotypic variation.Additionally,QCAT-6 D-2 on chromosome 6 D explained 16.80%of the phenotypic variation,with the positive effect contributed by Huapei-3.Furthermore,eight additive QTLs for MDA content were detected on chromosomes 1 A,2 B,3 A,4 B,5 B.2,6 B,and 7 B,including QMDA-6 B between markers Xcfa2257 and Xcfd48,which was responsible for 20.21%of the phenotypic variation.By marker-assisted selection,the major QTLs detected in this study may be valuable for improving freezing tolerancein wheat.更多还原