【作者】 王贵华； 李旭辉； 李莉； 顾日良； 王建华；

【Author】 WANG Gui-Hua;LI Xu-Hui;Li Li;GU Ri-Liang;WANG Jian-Hua;Center of Seed Science and Technology,College of Agronomy and Biotechnology,China Agricultural University,Beijing innovation center for seed technology,Ministry of Agriculture;

【机构】 中国农业大学农学院种子科学与技术研究中心农业部种子全程技术研究北京创新中心；

【摘要】 玉米是起源于热带亚热带地区的典型喜温作物,对低温较为敏感。在萌发阶段遭遇低温胁迫不但会降低玉米种子的出苗率和幼苗活力,还会增加被土壤病原菌侵染的几率,严重影响玉米产量。在10℃和25℃条件下分别测定了368份玉米自交系的低温发芽率和标准发芽率,利用分布于玉米全基因组的55万个单核苷酸多态性(Single Nucleotide Polymorphism;SNP)标记对相对低温发芽率进行全基因组关联分析(Genome-wide association study;GWAS),共获得32个显著的SNPs(P<0.0001)。全基因组关联分析可以快速有效地获得与玉米低温萌发特性显著关联的位点和候选基因,为相关基因的克隆与转化奠定基础,提高玉米的低温抗性;为研究玉米萌发阶段响应低温的分子机理提供帮助。更多还原

【Abstract】 Maize(Zea mays L.) is a typical warm temperate crop originating from tropical and subtropical regions,which is sensitive to low temperature.During the germination stage,low temperature stress will not only reduce the germination rate and seedling vigor of maize seeds,but also increase the probability of infection by soil pathogens,and seriously affect the yield of maize.Low germination rate and standard germination rate of 368 maize inbred lines was detected under theconditions of 10℃ and 25 ℃,respectively.550 thousand single nucleotide polymorphismmarkers were used to perform genome-wide association study for the relative low temperature germination rate and we found 32 significantly SNPs at a threshold of P<0.0001.These significantly SNPs lay a foundation for the cloning and transformation of related genes,then enhance the low temperature tolerance of Maize duringgermination stage;Help to study molecular mechanism of maize to response low temperature during seed germination.更多还原