白藜芦醇对大鼠动脉硬化后MMP-2及MMP-9蛋白的影响

【作者】 苏军华； 郭勇； 范琼英； 石桂芳； 华国祥；

【机构】 河北医科大学第二医院检验科； 石家庄市第一医院医务处； 石家庄市第一医院科研处； 冀中能源峰峰集团总医院； 邯郸陶瓷(集团)总公司医院；

【摘要】 目的研究与讨论白藜芦醇(resveratrol Res)对大鼠动脉硬粥样硬化(atherosclerosis,AS)基质金属蛋白酶(matrix metalloproteinase,MMPs)表达的影响。方法选择健康Wistar大鼠60只随机分为正常对照组、模型组、白藜芦醇组(Res组),每组样本各20只,大鼠动脉粥样硬化模型制备方法:液氮冻伤术,模型制备成功2个月后使用液氮冻伤术促使血管内硬化斑块破裂,于模型制备前、制备后2个月及激发试验后48h分别测定三组动物模型MMP-2,9以及总胆固醇(TC)、低密度脂蛋白(LDL)、甘油三酯(TG)的浓度。结果模型组造模后2个月MMP-2,9的浓度分别为(50.64±6.49)ng/ml和(338.79±9.98)ng/ml,同时期Res组MMP-2,9的浓度分别为(40.33±5.58)ng/ml和(304.53±7.59)ng/ml(P<0.05),激发试验48h后模型组M M P-2,9的浓度分别为(101.45±5.58)ng/ml和(859.56±11.3)ng/ml,同期Res组MMP-2,9的浓度分别为(60.68±1 2.69)ng/ml和(401.24±15.3)ng/ml(P<0.05)。结论白藜芦醇(resveratrol Res)可通过抑制MMP-2,9的表达,对动脉粥样硬化的发生、发展起到预防和延缓的作用。更多还原

【Abstract】 Objective To study the effect of resveratrol on expression of matrix metalloproteinase(MMPs) in rat with experimental atherosclerosis.Methods Sixty Wistar healthy rats were randomly divided into normal control group(n=20).atherosclerosis model group(n = 20),and resveratrol group(n=20).Two months after an atherosclerosis model was established using liquid nitrogen,cold injury was induced to provoke rupture of atherosclerosis plaque.levels of MMP-2 and 9 were measured before model establishment.2 months after model establishment and 48 hours after provocation test,respectively.Results The levels of MMP-2 and 9were(50.64±6.49)ng/ml and(338.79±9.98)ng/ml in model group and(40.33±5.58)ng/ml and(304.53±7.59)ng/ml in resveratrol group.respectively,2 months after model establishment(P<0.05),while they were(101.45±5.58)ng/ml and(859.56±11.3) ng/ml in model group and(60.68± 12.69) ng/ml and(401.24 ± 15.3) ng/ml in resveratrol group,respectively,48 hours after provocation test(P<0.05).Conclusion Resveratrol can prevent and delay the occurrence of atherosclerosis by reducing the level of MMP-2 and MMP-9.更多还原